Wong R L, Lingenheld E G, Fitzgerald L, Clark R B
Department of Medicine, University of Connecticut Health Center, Farmington 06032.
Cell Immunol. 1989 Oct 15;123(2):445-55. doi: 10.1016/0008-8749(89)90304-3.
Granulocyte-macrophage colony-stimulating factor (GmCSF) is a lymphokine secreted by class II major histocompatibility complex (MHC)-restricted T cells after lectin or antigen stimulation. To investigate the relationship between interleukin-2 (IL-2) and GmCSF production, we utilized long-term cultures of porcine myelin basic protein (PMBP)-specific T helper cell clones that were maintained with IL-2 in the absence of antigen or irradiated antigen-presenting cells (APC). We have found that supernatants of these T cell clones contained GmCSF activity after IL-2 stimulation. Inhibition of cell proliferation by irradiation failed to stop GmCSF production. When these clones were stimulated with PMBP and irradiated APC in the presence of anti-IL-2 receptor antibody, the T cell supernatants still contained GmCSF activity. These results indicate that (1) GmCSF production by T helper clones after IL-2 stimulation is independent of cell proliferation and (2) antigen/MHC-stimulated GmCSF production by T cell clones can occur by an IL-2-independent pathway.
粒细胞巨噬细胞集落刺激因子（GmCSF）是一种由II类主要组织相容性复合体（MHC）限制的T细胞在凝集素或抗原刺激后分泌的淋巴因子。为了研究白细胞介素-2（IL-2）与GmCSF产生之间的关系，我们利用了猪髓磷脂碱性蛋白（PMBP）特异性T辅助细胞克隆的长期培养物，这些克隆在没有抗原或经照射的抗原呈递细胞（APC）的情况下用IL-2维持培养。我们发现，在IL-2刺激后，这些T细胞克隆的上清液含有GmCSF活性。照射抑制细胞增殖并不能阻止GmCSF的产生。当这些克隆在抗IL-2受体抗体存在的情况下用PMBP和经照射的APC刺激时，T细胞上清液仍含有GmCSF活性。这些结果表明：（1）IL-2刺激后T辅助克隆产生GmCSF与细胞增殖无关；（2）T细胞克隆经抗原/MHC刺激产生GmCSF可通过一条不依赖IL-2的途径发生。
