Bloemena E, Roos M T, Van Heijst J L, Vossen J M, Schellekens P T
Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
J Immunol Methods. 1989 Sep 1;122(2):161-7. doi: 10.1016/0022-1759(89)90260-3.
A simple and reproducible method is described for the measurement of proliferative responses of peripheral blood lymphocytes in whole blood upon stimulation with horse anti-human lymphocyte serum (ALS), phytohaemagglutinin (PHA) or a monoclonal antibody (mAb) directed against CD3. Only small aliquots of blood are needed and separation procedures are not necessary. Since the method proved to be very reproducible and showed little variation, reference values of proliferation, expressed in cpm per 15 microliters blood and cpm per 10(3) CD3+ lymphocytes, were determined based upon the 5th and the 2.5th percentile of the distribution of the values in our reference population. In addition, the use of whole blood lymphocyte cultures in longitudinal studies of immunocompromised individuals is demonstrated.
本文描述了一种简单且可重复的方法，用于测量全血中经马抗人淋巴细胞血清（ALS）、植物血凝素（PHA）或抗CD3单克隆抗体（mAb）刺激后外周血淋巴细胞的增殖反应。仅需少量血样，无需分离程序。由于该方法具有高度可重复性且变异性小，因此根据我们参考人群中数值分布的第5百分位数和第2.5百分位数，确定了以每15微升血液的cpm数和每10³个CD3⁺淋巴细胞的cpm数表示的增殖参考值。此外，还展示了全血淋巴细胞培养在免疫功能低下个体纵向研究中的应用。
