Moshourab Rabih, Schmidt Yvonne, Machelska Halina
Klinik für Anästhesiologie und Operative Intensivmedizin, Freie Universität Berlin, Charité-Universitätsmedizin Berlin, Campus Mitte and Virchow-Klinikum, Augustenburger Platz 1, Berlin, 13353, Germany,
Methods Mol Biol. 2015;1230:215-28. doi: 10.1007/978-1-4939-1708-2_17.
This chapter describes the methodology of the in vitro skin-saphenous nerve preparation and its application to test for the modulatory effects of opioids on the function of cutaneous sensory neurons in experimental models of pain. We detail the skin-nerve setup requirements and the technique to record action potentials from single sensory fibers. We address how to test for inhibitory effects of opioid receptor activation on mechanical and thermal sensitivity of nociceptors and mechanoreceptors in the complete Freund's adjuvant-induced inflammation and the chronic constriction injury model of neuropathic pain.
本章描述了体外皮肤 - 隐神经制备方法及其在疼痛实验模型中用于测试阿片类药物对皮肤感觉神经元功能调节作用的应用。我们详细介绍了皮肤 - 神经设置要求以及从单根感觉纤维记录动作电位的技术。我们阐述了在完全弗氏佐剂诱导的炎症和神经性疼痛的慢性压迫损伤模型中，如何测试阿片受体激活对伤害感受器和机械感受器的机械和热敏感性的抑制作用。
