Dormans J A
Laboratory for Pathology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
J Microsc. 1989 Aug;155(Pt 2):207-11. doi: 10.1111/j.1365-2818.1989.tb02883.x.
Alveolar type II cells in control and ozone-exposed rat lungs were counted at the light microscopical level with the 'disector method'. The type II cells were unequivocally marked by histochemical staining for alkaline phosphatase activity in 2 microns plastic sections. By this counting method, the mean number of type II cells per lung in control rats was of the same magnitude as those reported in the literature, using point counting methods. After exposure of rats to 1.6 mg ozone/m3 for 7 days, a 50% increase in the mean number of type II cells was observed. The use of the disector method at the light microscopical level offers some advantages above a quantification at the electron microscopical level. The procedure is less time-consuming, larger areas can be screened, two parallel countings can be performed in one set of sections and there is no need for an exact knowledge about the diameter of the measured particle.
采用“分割法”在光学显微镜水平对对照大鼠和暴露于臭氧环境的大鼠肺组织中的Ⅱ型肺泡细胞进行计数。通过对2微米厚的塑料切片进行碱性磷酸酶活性的组织化学染色，Ⅱ型细胞得到明确标记。采用这种计数方法，对照大鼠每只肺中Ⅱ型细胞的平均数量与文献中使用点计数法报道的数量处于同一量级。将大鼠暴露于1.6毫克臭氧/立方米环境7天后，观察到Ⅱ型细胞平均数量增加了50%。在光学显微镜水平使用分割法比在电子显微镜水平进行定量分析具有一些优势。该过程耗时较少，可以筛查更大的区域，在一组切片中可以进行两次平行计数，并且无需精确了解所测颗粒的直径。
