Ivashchenko Sergiy, Escobar Ivirico Jorge L, García Cruz Dunia M, Campillo-Fernández Alberto, Gallego Ferrer Gloria, Monleón Pradas Manuel
Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, Spain
Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, Spain Present address: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
J Biomater Appl. 2015 Mar;29(8):1096-108. doi: 10.1177/0885328214554816. Epub 2014 Oct 6.
A series of novel poly(CLMA-co-HEA)/silica nanocomposites is synthesized from caprolactone 2-(methacryloyloxy)ethyl ester (CLMA) and 2-hydroxyethyl acrylate (HEA) as organic comonomers and the simultaneous sol-gel polymerization of tetraethyloxysilane (TEOS) as silica precursor, in different mass ratios up to a 30 wt% of silica. The nanocomposites are characterized as to their mechanical and thermal properties, water sorption, bioactivity and biocompatibility, reflecting the effect on the organic matrix provided by the silica network formation. The nanocomposites nucleate the growth of hydroxyapatite (HAp) on their surfaces when immersed in the simulated body fluid of the composition used in this work. Proliferation of the MC3T3 osteoblast-like cells on the materials was assessed with the MTS assay showing their biocompatibility. Immunocytochemistry reveals osteocalcin and type I collagen production, indicating that osteoblast differentiation was promoted by the materials, and calcium deposition was confirmed by von Kossa staining. The results indicate that these poly(CLMA-co-HEA)/silica nanocomposites could be a promising biomaterial for bone tissue engineering.
以己内酯 2-(甲基丙烯酰氧基)乙酯(CLMA)和丙烯酸 2-羟乙酯(HEA)作为有机共聚单体，同时以正硅酸乙酯(TEOS)作为二氧化硅前驱体进行溶胶-凝胶聚合，合成了一系列新型的聚(CLMA-co-HEA)/二氧化硅纳米复合材料，二氧化硅的质量比最高可达 30 wt%。对纳米复合材料的力学性能、热性能、吸水性、生物活性和生物相容性进行了表征，反映了二氧化硅网络形成对有机基体的影响。当将纳米复合材料浸入本研究中使用的模拟体液中时，它们会在其表面使羟基磷灰石(HAp)生长成核。用 MTS 法评估了 MC3T3 成骨样细胞在材料上的增殖情况，显示了它们的生物相容性。免疫细胞化学揭示了骨钙素和 I 型胶原蛋白的产生，表明材料促进了成骨细胞的分化，并且通过冯·科萨染色证实了钙沉积。结果表明，这些聚(CLMA-co-HEA)/二氧化硅纳米复合材料可能是骨组织工程中有前景的生物材料。
