Chamon Raiane Cardoso, Iorio Natalia Lopes Pontes, Cavalcante Fernanda Sampaio, Teodoro Cristiane R S, de Oliveira Ana Paula Chaves, Maia Fernanda, dos Santos Kátia Regina Netto
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Universidade Federal Fluminense, Rio de Janeiro, Brazil.
Diagn Microbiol Infect Dis. 2014 Dec;80(4):307-10. doi: 10.1016/j.diagmicrobio.2014.09.011. Epub 2014 Sep 19.
In this work, the molecular and phenotypic antimicrobial resistance and clonal diversity of 10 linezolid-resistant Staphylococcus spp. isolates were investigated. The 7 Staphylococcus haemolyticus isolates presented Staphylococcal cassete chromosome mec (SCCmec) V and belonged to the same pulsed-field gel electrophoresis pulsotype. Their MICs for oxacillin, vancomycin, and linezolid were ≥ 256 μg/mL, 1-4 μg/mL, and 8-16 μg/mL, respectively. The 3 S. hominis presented MIC values 32 to >256 μg/mL, 2-4 μg/mL, and 12-24 μg/mL, and all carried the nontypeable SCCmec (ccr1 + mecA class) and belonged to 2 different genotypes. The cfr gene was not found, but the mutation G2603T was detected in S. haemolyticus and C2190T and G2603T in Staphylococcus hominis in 23S rRNA. This study demonstrates the spread of a linezolid-resistant S. haemolyticus genotype and, for the first time, describes the mutation C2190T among S. hominis isolates with a double mutation in Brazil.
在本研究中，对10株耐利奈唑胺葡萄球菌属分离株的分子和表型抗菌药物耐药性及克隆多样性进行了研究。7株溶血葡萄球菌分离株携带葡萄球菌盒式染色体mec（SCCmec）V，属于同一脉冲场凝胶电泳脉冲型。它们对苯唑西林、万古霉素和利奈唑胺的最低抑菌浓度（MIC）分别≥256μg/mL、1 - 4μg/mL和8 - 16μg/mL。3株人葡萄球菌的MIC值分别为32至>256μg/mL、2 - 4μg/mL和12 - 24μg/mL，均携带不可分型的SCCmec（ccr1 + mecA类），属于2种不同基因型。未发现cfr基因，但在溶血葡萄球菌中检测到23S rRNA的G2603T突变，在人葡萄球菌中检测到C2190T和G2603T突变。本研究证明了耐利奈唑胺溶血葡萄球菌基因型的传播，并首次描述了巴西人葡萄球菌分离株中存在的C2190T突变及双重突变。
