榕树枝干甲醇提取物对四氧嘧啶诱导的糖尿病大鼠的降血脂和血液学评价

Hypolipidaemic and haematological evaluation of the hydromethanolic extract of Ficus glumosa stem bark in alloxan-induced diabetic rats.

作者信息

Onoja Samuel O, Omeh Yusuf N, Ezeja Maxwell I, Chukwu Vincent N

出版信息

J Complement Integr Med. 2014 Dec;11(4):259-64. doi: 10.1515/jcim-2014-0029.

DOI:10.1515/jcim-2014-0029

PMID:25294718

Abstract

BACKGROUND

Diabetes mellitus is associated with dyslipidaemia and haematological disorders which lead to cardiovascular complications and anaemia respectively. In this study, hydromethanolic extract of Ficus glumosa was evaluated for its hypolipidaemic and haematological effects in alloxan-induced diabetic rats.

METHODS

Treatment was done orally using 62.5, 125 and 250 mg/kg of F. glumosa extract (FGE) with glibenclamide (2 mg/kg) as standard reference drug for 21 days. At the end of the treatment, blood collected through direct heart puncture was used for evaluation of serum lipid profile and determination of haematological parameters. Organosomatic index evaluation of the liver, spleen and kidney was also done using standard procedures.

RESULTS

All doses of the extract and glibenclamide (2 mg/kg) caused dose-dependent and significant (p<0.05) reduction in serum levels of total cholesterol and low-density lipoprotein cholesterol (LDL-C) of the treated rats when compared with the control group. The extract and glibenclamide produced significant (p<0.05) increase in the serum levels of high-density lipoprotein cholesterol (HDL-C) in the treated rats. The extract (62.5 mg/kg) and glibenclamide (2 mg/kg) caused a significant (p<0.05) increase in red blood cell (RBC), haemoglobin (Hb), packed cell volume (PCV) and mean corpuscular haemoglobin concentration (MCHC) in the treated rats when compared to the negative control group. However, FGE (62.5 mg/kg) and glibenclamide (2 mg/kg) produced a significant (p<0.05) decrease in mean corpuscular volume (MCV) value of the treated rats. FGE (125 and 250 mg/kg) produced a significant (p<0.05) increase in the organosomatic index of kidney in treated rats.

CONCLUSIONS

The results suggest that F. glumosa may be of help in diabetes-induced anaemia and dyslipidaemia associated with diabetes mellitus.

摘要

背景

糖尿病与血脂异常和血液系统疾病相关，分别导致心血管并发症和贫血。在本研究中，对糙叶榕的水甲醇提取物在四氧嘧啶诱导的糖尿病大鼠中的降血脂和血液学作用进行了评估。

方法

以格列本脲（2 mg/kg）作为标准参考药物，使用62.5、125和250 mg/kg的糙叶榕提取物（FGE）进行口服给药，持续21天。治疗结束时，通过直接心脏穿刺采集的血液用于评估血清脂质谱和测定血液学参数。还使用标准程序对肝脏、脾脏和肾脏进行了器官体指数评估。

结果

与对照组相比，所有剂量的提取物和格列本脲（2 mg/kg）均导致治疗大鼠血清总胆固醇和低密度脂蛋白胆固醇（LDL-C）水平呈剂量依赖性且显著降低（p<0.05）。提取物和格列本脲使治疗大鼠血清高密度脂蛋白胆固醇（HDL-C）水平显著升高（p<0.05）。与阴性对照组相比，提取物（62.5 mg/kg）和格列本脲（2 mg/kg）使治疗大鼠的红细胞（RBC）、血红蛋白（Hb）、血细胞比容（PCV）和平均红细胞血红蛋白浓度（MCHC）显著增加（p<0.05）。然而，FGE（62.5 mg/kg）和格列本脲（2 mg/kg）使治疗大鼠的平均红细胞体积（MCV）值显著降低（p<0.05）。FGE（125和250 mg/kg）使治疗大鼠肾脏的器官体指数显著增加（p<0.05）。