Cooke M, Tan E K, Mandrycky C, He H, O'Connell J, Tseng S C G
Amniox Medical, Atlanta, GA, 30339, USA;
J Wound Care. 2014 Oct;23(10):465-74, 476. doi: 10.12968/jowc.2014.23.10.465.
To evaluate how the different processing methods cryopreservation and dehydration affect the structural integrity and biological composition of key signalling molecules within amniotic membrane and umbilical cord tissues.
We directly compared cryopreserved amniotic membrane (AM) and umbilical cord (UC) tissues with dehydrated amniotic membrane/chorion (dHACM) tissue using biochemical and functional assays including histological and histochemical staining, BCA, agarose gel electrophoresis, western blot, ELISA, and proliferation and cell death assays.
Cryopreservation retains the native architecture of the AM/UC extracellular matrix and maintains the quantity and activity of key biological signals present in fresh AM/UC, including high molecular weight hyaluronic acid, heavy chain-HA complex, and pentraxin 3. In contrast, dehydrated tissues were structurally compromised and almost completely lacked these crucial components.
The results presented here indicate that cryopreservation better preserves the structural and biological signaling molecules of foetal tissues.
评估不同处理方法（冷冻保存和脱水）如何影响羊膜和脐带组织内关键信号分子的结构完整性和生物组成。
我们使用生化和功能分析方法，包括组织学和组织化学染色、BCA、琼脂糖凝胶电泳、蛋白质印迹法、酶联免疫吸附测定以及增殖和细胞死亡测定，将冷冻保存的羊膜（AM）和脐带（UC）组织与脱水羊膜/绒毛膜（dHACM）组织进行直接比较。
冷冻保存保留了AM/UC细胞外基质的天然结构，并维持了新鲜AM/UC中存在的关键生物信号的数量和活性，包括高分子量透明质酸、重链 - HA复合物和五聚素3。相比之下，脱水组织在结构上受到损害，几乎完全缺乏这些关键成分。
此处呈现的结果表明，冷冻保存能更好地保留胎儿组织的结构和生物信号分子。
