Kondeva-Burdina Magdalena, Krasteva Ilina, Mitcheva Mitka
Laboratory for Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Sofia, Bulgaria.
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, Sofia, Bulgaria.
Pharmacogn Mag. 2014 Aug;10(Suppl 3):S487-93. doi: 10.4103/0973-1296.139778.
Astragalus hamosus L. (Fabaceae) is used in herbal medicine as emollient, demulcent, phrodisiac, diuretic, laxative, and good for inflammation, ulcers, and leukoderma. It is useful in treating irritation of the mucous membranes, nervous affections, and catarrh.
Rhamnocitrin 4-β-D-galactopyranoside (RGP), isolated from A. hamosus, was investigated for its possible protective effect on different models of toxicity in vitro on sub-cellular and cellular level.
The effects of RGP were evaluated on isolated rat brain synaptosomes, prepared by Percoll reagent and on rat hepatocytes, isolated by two-stepped collagenase perfusion.
In synaptosomes, RGP had statistically significant protective effect, similar to those of silymarin, on 6-hydroxy (OH)-dopamine-induced oxidative stress. These results correlate with literature data about protective effects of kempferol and rhamnocitrin on oxidative damage in rat pheochromocytoma PC12 cells. In rat hepatocytes, we investigate the effect of RGP on two models of liver toxicity: Bendamustine and cyclophosphamide. In these models, the compound had statistically significant cytoprotective and antioxidant activity, similar to those of silymarin.
According to these results, we can suggest that such cytoprotective effect of RGP might be due to an influence on bendamustine and cyclophosphamide metabolism in rat hepatocytes. In isolated rat hepatocytes, in combination with bendamustine and cyclophosphamide and in 6-OH-dopamine-induced oxidative stress in isolated rat synaptosomes, RGP, isolated from A. hamosus, was effective protector and antioxidant. The effects were closed to those of flavonoid silymarin-the classical hepatoprotector and antioxidant.
鹰嘴紫云英（豆科）在草药医学中用作润肤剂、缓和剂、催情剂、利尿剂、泻药，对炎症、溃疡和白斑病有益。它可用于治疗粘膜刺激、神经疾病和卡他。
研究从鹰嘴紫云英中分离得到的鼠李柠檬素4-β-D-吡喃半乳糖苷（RGP）在体外亚细胞和细胞水平对不同毒性模型的可能保护作用。
通过Percoll试剂制备的大鼠脑突触体和两步胶原酶灌注分离的大鼠肝细胞，评估RGP的作用。
在突触体中，RGP对6-羟基（OH）-多巴胺诱导的氧化应激具有统计学上显著的保护作用，与水飞蓟宾的作用相似。这些结果与文献中关于山奈酚和鼠李柠檬素对大鼠嗜铬细胞瘤PC12细胞氧化损伤保护作用的数据相关。在大鼠肝细胞中，我们研究了RGP对两种肝毒性模型的作用：苯达莫司汀和环磷酰胺。在这些模型中，该化合物具有统计学上显著的细胞保护和抗氧化活性，与水飞蓟宾的作用相似。
根据这些结果，我们可以认为RGP的这种细胞保护作用可能是由于对大鼠肝细胞中苯达莫司汀和环磷酰胺代谢的影响。在分离的大鼠肝细胞中，与苯达莫司汀和环磷酰胺联合使用时，以及在分离的大鼠突触体中6-OH-多巴胺诱导的氧化应激中，从鹰嘴紫云英中分离得到的RGP是有效的保护剂和抗氧化剂。其作用与经典的肝保护剂和抗氧化剂黄酮类水飞蓟宾的作用相近。
