Fan X C, Chu J Q, Liu C H, Sun X, Fang J G
Zhengzhou Fruit Research Institute, Chinese Academy of Agriculture Science, Zhengzhou China
College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Genet Mol Res. 2014 Sep 26;13(3):7649-57. doi: 10.4238/2014.September.26.2.
Grapevine (Vitis) rootstock varieties or cultivars are used to confer resistance and tolerance to insect and disease pests, unfavorable soil conditions, and other environmental conditions to cultivars that are susceptible to these conditions but otherwise have desired properties. The need to genotype and thoroughly identify grapevine rootstock varieties in the grape industry has become increasingly critical as more and more varieties are bred or selected. Although DNA markers have advantageous applications in plant identification, markers developed from classic DNA fingerprint analysis methods are not practical for plant cultivar identification. The manual cultivar identification diagram (MCID), which was previously developed in our research group, has been shown to select DNA markers that are relatively more exploitable in identifications of genotyped plant individuals. Using this MCID strategy and expressed sequence tag-simple sequence repeat (EST-SSR) markers, we identified 22 grapevine rootstock cultivars of diverse origin. All cultivars were clearly separated by fingerprints of seven pairs of EST-SSR primers and the grapevine rootstock CID (V-R-CID) generated is both practical and referable for the identification of any grapevine rootstock cultivars studied here. Furthermore, fewer primers can be used to distinguish all cultivars using this approach since the fingerprint from each primer pair could be used several times once it is generated. This initial version of V-R-CID can be made more informative with the identification and incorporation of more cultivars, thus providing better service to the grape industry.
葡萄（葡萄属）砧木品种被用于赋予对病虫害、不利土壤条件及其他环境条件具有抗性和耐受性的特性，这些特性原本存在于易受这些条件影响但具有理想性状的栽培品种中。随着越来越多的葡萄品种被培育或选育出来，在葡萄产业中对葡萄砧木品种进行基因分型和全面鉴定的需求变得越来越关键。尽管DNA标记在植物鉴定中具有优势应用，但从经典DNA指纹分析方法开发的标记对于植物品种鉴定并不实用。我们研究小组之前开发的人工品种鉴定图（MCID）已被证明能够选择在基因分型植物个体鉴定中相对更具可利用性的DNA标记。利用这种MCID策略和表达序列标签-简单序列重复（EST-SSR）标记，我们鉴定了22个不同来源的葡萄砧木品种。所有品种都通过七对EST-SSR引物的指纹图谱清晰区分，并且所生成的葡萄砧木CID（V-R-CID）对于鉴定本文研究的任何葡萄砧木品种既实用又具有参考价值。此外，使用这种方法可以用更少的引物区分所有品种，因为一旦生成，每个引物对的指纹图谱可以多次使用。随着更多品种的鉴定和纳入，V-R-CID的这个初始版本可以变得更具信息性，从而为葡萄产业提供更好的服务。
