利用ERIC-PCR标记分析巴西香蕉叶斑病菌的遗传多样性。

Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker.

作者信息

Silva G F, Paixão R D V, Queiroz C B, Santana M F, Souza A, Sousa N R, Hanada R E, Gasparotto L

机构信息

Laboratório de Biologia Molecular, Embrapa Amazônia Ocidental, Manaus, AM, Brasil

Laboratório de Biologia Molecular, Embrapa Amazônia Ocidental, Manaus, AM, Brasil.

出版信息

Genet Mol Res. 2014 Sep 26;13(3):7698-707. doi: 10.4238/2014.September.26.7.

DOI:10.4238/2014.September.26.7

PMID:25299083

Abstract

The Enterobacterial repetitive intergenic consensus (ERIC) marker was used to analyze the genetic variability of Mycosphaerella fijiensis, the causative agent of Black Sigatoka disease in banana plants. A total of 123 isolates were used, which were divided into populations based on their original hosts and collection sites in Brazil. A total of 9 loci were amplified, 77.8% of which were found to be polymorphic. The genetic diversity found in the population was 0.20. Analysis of molecular variance (AMOVA) demonstrated that the highest level of genetic variation is within populations. Cluster analysis revealed three main groups in Brazil, with no correlation between geographic and genetic distance.

摘要

肠杆菌重复基因间共有序列（ERIC）标记用于分析香蕉黑叶斑病病原菌斐济球腔菌的遗传变异性。共使用了123个分离株，这些分离株根据其在巴西的原始寄主和采集地点分为不同群体。共扩增出9个位点，其中77.8%为多态性位点。群体中的遗传多样性为0.20。分子方差分析（AMOVA）表明，遗传变异的最高水平存在于群体内部。聚类分析揭示了巴西的三个主要群体，地理距离与遗传距离之间没有相关性。