Nawaz Muhammad, Arayne Muhammad Saeed, Sultana Najma, Abbas Hira Fatima
Department of Chemistry, United Arab Emirates University, Al-Ain 15551, United Arab Emirates.
Department of Chemistry, University of Karachi, Karachi 75270, Pakistan.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Feb 25;137:1050-4. doi: 10.1016/j.saa.2014.08.152. Epub 2014 Oct 6.
This work describes a RP-HPLC method for the determination and interaction studies of cefpirome with ACE-inhibitors (captopril, enalapril and lisinopril) in various buffers. The separation and interaction of cefpirome with ACE-inhibitors was achieved on a Purospher Star, C18 (5 μm, 250×4.6 mm) column. Mobile phase consisted of methanol: water (80:20, v/v, pH 3.3); however, for the separation of lisinopril, it was modified to methanol-water (40:60, v/v, pH 3.3) and pumped at a flow rate of 1 mL min(-1). In all cases, UV detection was performed at 225 nm. Interactions were carried out in physiological pH i.e., pH 1 (simulated gastric juice), 4 (simulated full stomach), 7.4 (blood pH) and 9 (simulated GI), drug contents were analyzed by reverse phase high performance liquid chromatography. Method was found linear in the concentration range of 1.0-50.0 μg mL(-1) with correlation coefficient (r(2)) of 0.999. Precision (RSD%) was less than 2.0%, indicating good precision of the method and accuracy was 98.0-100.0%. Furthermore, cefpirome-ACE-inhibitors' complexes were also synthesized and results were elucidated on the basis of FT-IR, and (1)H NMR. The interaction results show that these interactions are pH dependent and for the co-administration of cefpirome and ACE-inhibitors, a proper interval should be given.
本研究描述了一种反相高效液相色谱（RP-HPLC）方法，用于在各种缓冲液中测定头孢匹罗与血管紧张素转换酶抑制剂（卡托普利、依那普利和赖诺普利）并研究它们之间的相互作用。头孢匹罗与血管紧张素转换酶抑制剂的分离及相互作用是在Purospher Star C18（5μm，250×4.6mm）色谱柱上实现的。流动相由甲醇：水（80:20，v/v，pH 3.3）组成；然而，为了分离赖诺普利，流动相改为甲醇-水（40:60，v/v，pH 3.3），并以1 mL min⁻¹的流速泵送。在所有情况下，均在225nm处进行紫外检测。相互作用在生理pH值下进行，即pH 1（模拟胃液）、4（模拟全胃）、7.4（血液pH值）和9（模拟胃肠道），药物含量通过反相高效液相色谱进行分析。该方法在1.0 - 50.0μg mL⁻¹的浓度范围内呈线性，相关系数（r²）为0.999。精密度（相对标准偏差，RSD%）小于2.0%，表明该方法具有良好的精密度，准确度为98.0 - 100.0%。此外，还合成了头孢匹罗 - 血管紧张素转换酶抑制剂复合物，并基于傅里叶变换红外光谱（FT-IR）和核磁共振氢谱（¹H NMR）对结果进行了阐释。相互作用结果表明，这些相互作用依赖于pH值，对于头孢匹罗和血管紧张素转换酶抑制剂的联合给药，应给予适当的间隔时间。
