氨酰-tRNA合成酶突变体以正常速率降解蛋白质。
Aminoacyl-tRNA synthetase mutants degrade protein at a normal rate.
作者信息
Clark J L, Rabe J, Arfin S M
出版信息
J Cell Physiol. 1979 Jan;98(1):237-9. doi: 10.1002/jcp.1040980125.
PMID:253005
Abstract
The stability of both rapidly and slowly degraded proteins in wild type CHO cells is similar to that in three ts aminoacyl-tRNA synthetase mutants at both permissive and non-permissive temperatures, although the degree of tRNA charging in the synthetase mutants differs considerably with temperature. These results indicate that the altered rate of protein breakdown seen under a variety of physiological conditions in eukaryotic systems is not mediated by uncharged tRNA.
摘要
在野生型CHO细胞中，快速降解和缓慢降解的蛋白质的稳定性在允许温度和非允许温度下都与三种温度敏感型氨酰-tRNA合成酶突变体中的相似，尽管合成酶突变体中tRNA的负载程度随温度有很大差异。这些结果表明，在真核系统的各种生理条件下观察到的蛋白质分解速率的改变不是由未负载的tRNA介导的。
相似文献
1
Aminoacyl-tRNA synthetase mutants degrade protein at a normal rate.氨酰-tRNA合成酶突变体以正常速率降解蛋白质。
J Cell Physiol. 1979 Jan;98(1):237-9. doi: 10.1002/jcp.1040980125.
2
Relationship between histidyl-tRNA level and protein synthesis rate in wild-type and mutant Chinese hamster ovary cells.野生型和突变型中国仓鼠卵巢细胞中组氨酰-tRNA水平与蛋白质合成速率之间的关系。
J Cell Physiol. 1979 Jun;99(3):303-12. doi: 10.1002/jcp.1040990304.
3
[Mechanism of functioning of aminoacyl-tRNA-synthetases].[氨酰-tRNA合成酶的作用机制]
Mol Biol (Mosk). 1984 Sep-Oct;18(5):1264-86.
4
Use of the kinetic equilibrium between aminoacyl-tRNA formation and hydrolysis in inhibition assays of aminoacyl-tRNA synthetase.
Anal Biochem. 2001 Nov 1;298(1):133-6. doi: 10.1006/abio.2001.5387.
5
Altered aminoacyl-tRNA synthetase complexes in CHO cell mutants.CHO细胞突变体中改变的氨酰-tRNA合成酶复合物。
J Cell Physiol. 1983 Jan;114(1):82-7. doi: 10.1002/jcp.1041140114.
6
Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein.氨酰-tRNA合成酶的大分子组装：蛋白质-蛋白质相互作用的鉴定及一种核心蛋白的表征
J Mol Biol. 1999 Jan 8;285(1):183-95. doi: 10.1006/jmbi.1998.2316.
7
Characterization of cell lines showing growth control isolated from both the wild type and a leucyl-tRNA synthetase mutant of Chinese hamster ovary cells.从中国仓鼠卵巢细胞的野生型和亮氨酰 - tRNA合成酶突变体中分离出的显示生长控制的细胞系的特征描述。
J Cell Physiol. 1979 Mar;98(3):571-85. doi: 10.1002/jcp.1040980315.
8
A chimaeric glutamyl:glutaminyl-tRNA synthetase: implications for evolution.一种嵌合的谷氨酰胺：谷氨酰胺-tRNA合成酶：对进化的启示
Biochem J. 2009 Jan 15;417(2):449-55. doi: 10.1042/BJ20080747.
9
[Interaction of eukaryotic aminoacyl-tRNA-synthases with polyribosomes].[真核生物氨酰-tRNA合成酶与多核糖体的相互作用]
Vopr Med Khim. 1990 Sep-Oct;36(5):6-8.
10
Substrate selection by aminoacyl-tRNA synthetases.氨酰-tRNA合成酶对底物的选择
Nucleic Acids Symp Ser. 1995(33):40-2.
Zotero 插件