Igarashi K, Kashiwagi K, Kobayashi H, Ohnishi R, Kakegawa T, Nagasu A, Hirose S
Faculty of Pharmaceutical Sciences, Chiba University.
J Biochem. 1989 Aug;106(2):294-8. doi: 10.1093/oxfordjournals.jbchem.a122847.
The effect of polyamines on F1-ATPase catalyzed reactions has been studied through the use of submitochondrial particles and F1-ATPase. ATP degradation catalyzed by submitochondrial particles and F1-ATPase was inhibited by spermine and spermidine. Spermine's inhibition was much greater than spermidine's effect. In contrast, P1-ATP exchange and succinate dependent ATP synthesis catalyzed by submitochondrial particles were both stimulated by spermine. The inhibition of ATPase activity by polyamines probably occurs through polyamine's replacement of Mg2+ on ATP, for the following reasons. (a) The ATPase activity inhibited by spermine was partially recovered when Mg2+ was added. (b) Spermine bound to ATP and phospholipids but not to F1-ATPase; yet spermine inhibited the ATPase reaction catalyzed by F1-ATPase, a protein free of phospholipid. (c) The binding of spermine to ATP was inhibited by Mg2+. The ATP content in polyamine-deficient cells definitely was lower than that in normal cells. On the basis of these results, the possible role of spermine in keeping the ATP concentration at a high level is discussed.
通过使用亚线粒体颗粒和F1 - ATP酶，研究了多胺对F1 - ATP酶催化反应的影响。亚线粒体颗粒和F1 - ATP酶催化的ATP降解受到精胺和亚精胺的抑制。精胺的抑制作用远大于亚精胺。相反，亚线粒体颗粒催化的P1 - ATP交换和琥珀酸依赖性ATP合成均受到精胺的刺激。多胺对ATP酶活性的抑制可能是由于多胺取代了ATP上的Mg2 +，原因如下：(a) 添加Mg2 +时，被精胺抑制的ATP酶活性部分恢复。(b) 精胺与ATP和磷脂结合，但不与F1 - ATP酶结合；然而，精胺抑制了由不含磷脂的蛋白质F1 - ATP酶催化的ATP酶反应。(c) Mg2 +抑制精胺与ATP的结合。多胺缺乏细胞中的ATP含量肯定低于正常细胞。基于这些结果，讨论了精胺在将ATP浓度维持在高水平方面的可能作用。
