Chetwynd Andrew J, David Arthur, Hill Elizabeth M, Abdul-Sada Alaa
School of Life Sciences, University of Sussex, Falmer, Brighton, BN1 9QG, UK.
J Mass Spectrom. 2014 Oct;49(10):1063-9. doi: 10.1002/jms.3426.
Mass spectrometry (MS) profiling techniques are used for analysing metabolites and xenobiotics in biofluids; however, detection of low abundance compounds using conventional MS techniques is poor. To counter this, nanoflow ultra-high-pressure liquid chromatography-nanoelectrospray ionization-time-of-flight MS (nUHPLC-nESI-TOFMS), which has been used primarily for proteomics, offers an innovative prospect for profiling small molecules. Compared to conventional UHPLC-ESI-TOFMS, nUHPLC-nESI-TOFMS enhanced detection limits of a variety of (xeno)metabolites by between 2 and 2000-fold. In addition, this study demonstrates for the first time excellent repeatability and reproducibility for analysis of urine and plasma samples using nUHPLC-nESI-TOFMS, supporting implementation of this platform as a novel approach for high-throughput (xeno)metabolomics.
质谱（MS）分析技术用于分析生物流体中的代谢物和外源性物质；然而，使用传统MS技术检测低丰度化合物的效果不佳。为了解决这一问题，主要用于蛋白质组学的纳流超高压液相色谱-纳电喷雾电离-飞行时间质谱（nUHPLC-nESI-TOFMS）为小分子分析提供了一个创新前景。与传统的UHPLC-ESI-TOFMS相比，nUHPLC-nESI-TOFMS将多种（异源）代谢物的检测限提高了2至2000倍。此外，本研究首次证明了使用nUHPLC-nESI-TOFMS分析尿液和血浆样品具有出色的重复性和再现性，支持将该平台作为高通量（异源）代谢组学的一种新方法加以应用。
