Parisi M G, Benenati G, Cammarata M
Marine Immunobiology Laboratory, Department of Stebicef, Animal Biology section, University of Palermo, Palermo, Italy.
J Fish Dis. 2015 Nov;38(11):967-76. doi: 10.1111/jfd.12309. Epub 2014 Oct 11.
The F-lectin, a fucose-binding protein found from invertebrates to ectothermic vertebrates, is the last lectin family to be discovered. Here, we describe effects of two different types of stressors, bacterial infection and confinement stress, on the modulation of European sea bass Dicentrarchus labrax (L.) F-lectin (DlFBL), a well-characterized serum opsonin, using a specific antibody. The infection of the Vibrio alginolyticus bacterial strain increased the total haemagglutinating activity during the 16-day testing period. The DlFBL value showed an upward regulation on the first, second and last days and underwent a slight downward regulation 4 days post-challenge. In contrast, the effect of confinement and density stress showed a decrease in the plasma concentration of lectin, ranging from 50% to 60% compared with the control. The modulation of DlFBL is in line with the hypothesis that humoral lectins could be involved and recruited in the initial recognition step of the inflammation, which leads to agglutination, and the activation of mechanisms responsible for killing of the pathogens.
F-凝集素是一种从无脊椎动物到变温脊椎动物中发现的岩藻糖结合蛋白，是最后一个被发现的凝集素家族。在此，我们使用特异性抗体，描述了两种不同类型的应激源——细菌感染和限制应激，对欧洲海鲈（Dicentrarchus labrax，L.）F-凝集素（DlFBL）调节的影响，DlFBL是一种特征明确的血清调理素。在16天的测试期内，溶藻弧菌菌株感染增加了总血凝活性。DlFBL值在第1天、第2天和最后一天呈上调，在攻毒后4天略有下调。相比之下，限制和密度应激的影响显示凝集素的血浆浓度降低，与对照组相比降低了50%至60%。DlFBL的调节符合体液凝集素可能参与并被招募到炎症初始识别步骤的假设，这会导致凝集，并激活负责杀死病原体的机制。
