Faculty of Chemistry, University of Warsaw, ul. Pasteura 1, PL-02-093 Warsaw, Poland.
Faculty of Pharmacy, Medical University of Warsaw, ul. Banacha 1, PL-02-097 Warsaw, Poland.
Biosens Bioelectron. 2015 Feb 15;64:554-9. doi: 10.1016/j.bios.2014.09.079. Epub 2014 Oct 2.
An effective, fast, facile and direct electrochemical method of determination of hemoglobin (Hb) in blood sample without any sample preparation is described. The method is accomplished by using the ferromagnetic electrode modifier (carbon-encapsulated iron nanoparticles) and an external magnetic field. The successful voltammetric determination of hemoglobin is achieved in PBS buffer as well as in the whole blood sample. The obtained results show the excellent electroactivity of Hb. The measurements are of high sensitivity and good reproducibility. The detection limit is estimated to be 0.7 pM. The electrochemical determination data were compared with the gravimetric data obtained with a quartz crystal microbalance. The agreement between these results is very good. The changes of the electrode surface morphology before and after Hb detection are monitored by electron microscopy. The functionality of the electrochemical sensor is tested with human and rat blood samples. The concentration of hemoglobin in the blood samples determined by using voltammetric/gravimetric detection is in perfect agreement with the data obtained from typical clinical analysis.
本文描述了一种无需任何样品制备即可有效、快速、简便、直接地测定血液样品中血红蛋白（Hb）的电化学方法。该方法通过使用铁磁电极修饰剂（碳封装铁纳米粒子）和外加磁场来实现。成功地在 PBS 缓冲液以及全血样品中实现了血红蛋白的伏安测定。获得的结果表明 Hb 具有优异的电活性。测量具有高灵敏度和良好的重现性。检测限估计为 0.7 pM。将电化学测定数据与石英晶体微天平获得的重量法数据进行了比较。这些结果之间的一致性非常好。通过电子显微镜监测了 Hb 检测前后电极表面形貌的变化。通过使用人血和鼠血样品对电化学传感器的功能进行了测试。使用伏安/重量法检测确定的血液样品中的血红蛋白浓度与从典型临床分析获得的数据完全一致。
