Erdemir Umran Seven, Gucer Seref
Department of Chemistry, Faculty of Arts and Sciences, University of Uludag , Gorukle Campus, 16059 Nilufer-Bursa, Turkey.
J Agric Food Chem. 2014 Nov 5;62(44):10792-9. doi: 10.1021/jf503145t. Epub 2014 Oct 23.
In this study, an analytical fractionation scheme based on water, diethyl ether, n-hexane, and methanol extractions has been developed to identify manganese-bound fractions. Additionally, in vitro simulated gastric and intestinal digestion, n-octanol extraction, and activated carbon adsorption were used to interpret the manganese-bound structures in hazelnuts in terms of bioaccessibility. The total content of manganese in the samples was determined by inductively coupled plasma-mass spectrometry after microwave-assisted digestion, and additional validation was performed using atomic absorption spectroscopy. Water fractions were further evaluated by high-performance liquid chromatography hyphenated to inductively coupled plasma-mass spectrometry for the identification of water-soluble manganese fractions in hazelnut samples. The limits of detection and quantification were 3.6 and 12.0 μg L(-1), respectively, based on peak height.
