Greaney G S, Powers D A
J Exp Zool. 1978 Mar;203(3):339-50. doi: 10.1002/jez.1402030302.
Fundulus heteroclitus decreases erythrocyte adenosine triphosphate and increases blood hematocrit when acclimated to hypoxic conditions. A defined medium has been developed which allows isolated F. heteroclitus erythrocytes to be maintained for several hours without an appreciable loss of cellular ATP. The effect of oxygen tension, pH and metabolic inhibitors on the cellular concentration of ATP of fish red cells has been investigated as an in vitro model to explain in vivo responses to environmental changes. The isolated red cells significantly decrease their ATP/Hb molar ratio when exposed either to anaerobiosis or metabolic inhibitors. It is concluded that the in vivo response is mediated at the red cell level via decreased oxidative phosphorylation in the presence of low environmental oxygen. The length of time necessary to elicit the responce both in vivo and in vitro is also discussed.
当适应低氧环境时，底鳉会降低红细胞三磷酸腺苷水平并增加血液血细胞比容。已开发出一种特定培养基，可使分离的底鳉红细胞维持数小时而细胞三磷酸腺苷无明显损失。作为一种体外模型，研究了氧张力、pH值和代谢抑制剂对鱼红细胞三磷酸腺苷细胞浓度的影响，以解释体内对环境变化的反应。分离的红细胞在暴露于无氧环境或代谢抑制剂时，其三磷酸腺苷/血红蛋白摩尔比会显著降低。得出的结论是，体内反应是在红细胞水平上通过低环境氧存在下氧化磷酸化减少介导的。还讨论了在体内和体外引发反应所需的时间长度。
