细胞核中染色体定位的定量分析。

Quantitative analysis of chromosome localization in the nucleus.

作者信息

Chakraborty Sandeep, Mehta Ishita, Kulashreshtha Mugdha, Rao B J

机构信息

Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India,

出版信息

Methods Mol Biol. 2015;1228:223-33. doi: 10.1007/978-1-4939-1680-1_17.

DOI:10.1007/978-1-4939-1680-1_17

PMID:25311133

Abstract

The spatial organization of the genome within the interphase nucleus is important for mediating genome functions. The radial organization of chromosome territories has been studied traditionally using two-dimensional fluorescence in situ hybridization (FISH) using labeled whole chromosome probes. Information from 2D-FISH images is analyzed quantitatively and is depicted in the form of the spatial distribution of chromosomes territories. However, to the best of our knowledge no open-access tools are available to delineate the position of chromosome territories from 2D-FISH images. In this chapter we present a methodology termed Image Analysis of Chromosomes for computing their localization (IMACULAT). IMACULAT is an open-access, automated tool that partitions the cell nucleus into shells of equal area or volume and computes the spatial distribution of chromosome territories.

摘要

基因组在间期细胞核内的空间组织对于介导基因组功能至关重要。传统上，使用标记的全染色体探针通过二维荧光原位杂交（FISH）研究染色体区域的径向组织。对来自二维FISH图像的信息进行定量分析，并以染色体区域的空间分布形式进行描绘。然而，据我们所知，尚无开放获取的工具可用于从二维FISH图像中描绘染色体区域的位置。在本章中，我们介绍一种称为染色体图像分析以计算其定位（IMACULAT）的方法。IMACULAT是一种开放获取的自动化工具，可将细胞核划分为面积或体积相等的壳层，并计算染色体区域的空间分布。