Štramová X, Čegan A, Hampl R, Kanďár R
Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, Pardubice, Czech Republic.
Sanus Centre of Assisted Reproduction, Pardubice, Czech Republic.
Andrologia. 2015 Nov;47(9):967-73. doi: 10.1111/and.12365. Epub 2014 Oct 13.
The aim of this study was to investigate fatty acids composition of sperm phospholipids, level of lipoperoxidation represented by malondialdehyde and to examine differences between recent smokers and nonsmokers. The levels of malondialdehyde were in the group of all patients 1.51 ± 0.56 μmol l(-1) , in smokers 1.36 ± 0.59 μmol l(-1) and in nonsmokers 1.53 ± 0.55 μmol l(-1) . Total sperm membrane phospholipid fatty acids were profiled into several groups, saturated acids (in smokers 61.86 ± 9.02%, in nonsmokers 61.20 ± 11.66%), polyunsaturated acids n-3 (in smokers 12.62 ± 8.18%, in nonsmokers 14.28 ± 13.65%), polyunsaturated acids n-6 (in smokers 9.13 ± 4.37%, in nonsmokers 10.10 ± 3.79%) and other acids (in smokers 14.36 ± 3.94%, in nonsmokers 13.88 ± 2.31%). Significant correlations were found between the level of malondialdehyde (MDA) and total sperm motility in all patients (r = -0.358, P = 0.013), between both the level of MDA and progressive motility (r = -0.465, P = 0.001) and between the level of MDA and total motility (r = -0.382, P = 0.037) in nonsmokers. There were no statistically significant differences between composition of sperm phospholipid important fatty acids in smokers and nonsmokers. Significant correlations between selected sperm fatty acids and sperm motility and morphology in smokers and nonsmokers were not observed.
本研究的目的是调查精子磷脂的脂肪酸组成、以丙二醛表示的脂质过氧化水平，并检验近期吸烟者与非吸烟者之间的差异。所有患者组中丙二醛水平为1.51±0.56μmol l(-1)，吸烟者为1.36±0.59μmol l(-1)，非吸烟者为1.53±0.55μmol l(-1)。精子膜总磷脂脂肪酸被分为几组，饱和酸（吸烟者中为61.86±9.02%，非吸烟者中为61.20±11.66%）、多不饱和酸n-3（吸烟者中为12.62±8.18%，非吸烟者中为14.28±13.65%）、多不饱和酸n-6（吸烟者中为9.13±4.37%，非吸烟者中为10.10±3.79%）和其他酸（吸烟者中为14.36±3.94%，非吸烟者中为13.88±2.31%）。在所有患者中，丙二醛（MDA）水平与精子总活力之间存在显著相关性（r = -0.358，P = 0.013），在非吸烟者中，MDA水平与前向运动活力之间（r = -0.465，P = 0.001）以及MDA水平与总活力之间（r = -0.382，P = 0.037）均存在显著相关性。吸烟者与非吸烟者精子磷脂重要脂肪酸的组成之间无统计学显著差异。未观察到吸烟者与非吸烟者中所选精子脂肪酸与精子活力和形态之间的显著相关性。
