Li Duxin, Jakob Cornelia, Schmitz Oliver
Applied Analytical Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Anal Bioanal Chem. 2015 Jan;407(1):153-67. doi: 10.1007/s00216-014-8179-8. Epub 2014 Oct 14.
Online comprehensive two-dimensional liquid chromatography (online LCxLC) presents high peak capacity compared with one-dimensional liquid chromatography, and reasonable operation time compared with an offline or stop-flow mode. Among various combinations, coupling of two reversed-phase (RPxRP) columns generates a high peak capacity product rate. Its wide applicability made RPxRP a promising technique in separation of complex samples. This review discusses the practical considerations in development and application of an RPxRP system, including systematic investigation of column stationary phase chemistry, column combinations, mobile phase system, interface, gradient types, and achieving fast analysis in LCxLC. In addition, many efforts are given to methods that increase the fraction coverage because this is the main obstacle caused by the correlated separation mechanism in RPxRP.
在线全二维液相色谱（online LCxLC）与一维液相色谱相比具有较高的峰容量，与离线或停流模式相比具有合理的运行时间。在各种组合中，两根反相（RPxRP）柱的联用产生了较高的峰容量产物率。其广泛的适用性使RPxRP成为分离复杂样品的一种有前景的技术。本文综述讨论了RPxRP系统开发和应用中的实际考虑因素，包括对柱固定相化学、柱组合、流动相系统、接口、梯度类型的系统研究，以及在LCxLC中实现快速分析。此外，还对提高馏分覆盖率的方法进行了大量研究，因为这是RPxRP中相关分离机制导致的主要障碍。
