孔源性视网膜脱离患者眼内玻璃体α-B晶状体蛋白水平
Level of vitreous alpha-B crystallin in eyes with rhegmatogenous retinal detachment.
作者信息
Baba Takayuki, Oshitari Toshiyuki, Yamamoto Shuichi
机构信息
Department of Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260-0856, Japan,
出版信息
Graefes Arch Clin Exp Ophthalmol. 2015 Aug;253(8):1251-4. doi: 10.1007/s00417-014-2815-z. Epub 2014 Oct 14.
PURPOSE
Alpha-B crystallin (αB-crystallin) is a molecular chaperone, and one of the small heat shock proteins (sHSPs). αB-crystallin has been shown to have neuroprotective properties. The purpose of this study was to determine the level of αB-crystallin in eyes with a rhegmatogenous retinal detachment (RRD).
METHODS
Vitreous samples were collected before vitrectomy from 32 eyes of 32 consecutive patients with RRD. The concentration of αB-crystallin was measured by enzyme-linked immunosorbent assay (ELISA). Vitreous samples were also collected before vitrectomy from 16 patients with an idiopathic macular hole and epiretinal membrane, and used as controls.
RESULTS
The mean age was 56 years (range, 31-67 years) in the RRD cases and 66 years (range, 49-77 years) in the controls. The mean level of αB-crystallin was significantly higher in the RRD cases (36.7 ± 37.4 ng/ml) than that in controls (8.5 ± 12.2 ng/ml; P = 0.0028).
CONCLUSIONS
The increased level of αB-crystallin suggests that αB-crystallin may play a neuroprotective role by lessening retinal cell death after a retinal detachment.
目的
αB晶状体蛋白（αB-crystallin）是一种分子伴侣，也是小热休克蛋白（sHSPs）之一。已证明αB晶状体蛋白具有神经保护特性。本研究的目的是确定孔源性视网膜脱离（RRD）患者眼中αB晶状体蛋白的水平。
方法
对32例连续RRD患者的32只眼在玻璃体切除术前采集玻璃体样本。采用酶联免疫吸附测定（ELISA）法测定αB晶状体蛋白的浓度。还在玻璃体切除术前从16例特发性黄斑裂孔和视网膜前膜患者中采集玻璃体样本，并用作对照。
结果
RRD病例的平均年龄为56岁（范围31 - 67岁），对照组为66岁（范围49 - 77岁）。RRD病例中αB晶状体蛋白的平均水平（36.7±37.4 ng/ml）显著高于对照组（8.5±12.2 ng/ml；P = 0.0028）。
结论
αB晶状体蛋白水平升高表明αB晶状体蛋白可能通过减少视网膜脱离后视网膜细胞死亡而发挥神经保护作用。
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