增殖性糖尿病视网膜病变患者玻璃体液中αB-晶状体蛋白水平升高及其与血管内皮生长因子的相关性

Increased levels of alphaB-crystallin in vitreous fluid of patients with proliferative diabetic retinopathy and correlation with vascular endothelial growth factor.

作者信息

Chen Wei, Lu Qianyi, Lu Li, Guan Huaijin

机构信息

Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.

Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

出版信息

Clin Exp Ophthalmol. 2017 May;45(4):379-384. doi: 10.1111/ceo.12891. Epub 2017 Jan 27.

DOI:10.1111/ceo.12891

PMID:27928876

Abstract

BACKGROUND

AlphaB-crystallin has been shown to have angiogenic properties. The purpose of this study was to determine the levels of alphaB-crystallin in the vitreous fluid of patients with proliferative diabetic retinopathy and to confirm the association between the expression level of alphaB-crystallin and vascular endothelial growth factor.

METHODS

Vitreous samples were collected before vitrectomy from 46 eyes of 46 consecutive patients with proliferative diabetic retinopathy, and 19 patients without diabetes mellitus had vitrectomy for idiopathic macular hole. The concentrations of alphaB-crystallin and vascular endothelial growth factor were measured via enzyme-linked immunosorbent assay.

RESULTS

The vitreous level (mean ± SD) of alphaB-crystallin was significantly higher in patients with proliferative diabetic retinopathy (317.3 ± 151.7 ng/mL) than in control patients (idiopathic macular hole, 8.3 ± 6.1 ng/mL) (P < 0.0001). The vitreous concentration of vascular endothelial growth factor was also significantly higher in patients with proliferative diabetic retinopathy (860.1 ± 566.4 pg/mL) than in control patients (9 pg/mL) (P < 0.0001). Meanwhile, both the expression levels of alphaB-crystallin and vascular endothelial growth factor were significantly higher in eyes with active proliferative diabetic retinopathy than in those with inactive proliferative diabetic retinopathy. Also, the vitreous concentration of alphaB-crystallin correlated significantly with that of vascular endothelial growth factor in vitreous fluid of proliferative diabetic retinopathy ([correlation coefficient], R = 0.78, P < 0.001).

CONCLUSIONS

These results suggest a significant increase of alphaB-crystallin in the vitreous fluid of patients with proliferative diabetic retinopathy and present a crucial association between alphaB-crystallin and vascular endothelial growth factor with angiogenic activity in proliferative diabetic retinopathy.

摘要

背景

已证实αB-晶状体蛋白具有血管生成特性。本研究的目的是测定增殖性糖尿病视网膜病变患者玻璃体液中αB-晶状体蛋白的水平，并确认αB-晶状体蛋白表达水平与血管内皮生长因子之间的关联。

方法

从46例连续的增殖性糖尿病视网膜病变患者的46只眼中收集玻璃体样本，这些患者在玻璃体切除术前进行了样本采集，另外19例无糖尿病的患者因特发性黄斑裂孔接受了玻璃体切除术。通过酶联免疫吸附测定法测量αB-晶状体蛋白和血管内皮生长因子的浓度。

结果

增殖性糖尿病视网膜病变患者玻璃体内αB-晶状体蛋白水平（平均值±标准差）（317.3±151.7 ng/mL）显著高于对照组患者（特发性黄斑裂孔，8.3±6.1 ng/mL）（P<0.0001）。增殖性糖尿病视网膜病变患者玻璃体内血管内皮生长因子浓度（860.1±566.4 pg/mL）也显著高于对照组患者（9 pg/mL）（P<0.0001）。同时，活动性增殖性糖尿病视网膜病变眼的αB-晶状体蛋白和血管内皮生长因子表达水平均显著高于非活动性增殖性糖尿病视网膜病变眼。此外，增殖性糖尿病视网膜病变患者玻璃体液中αB-晶状体蛋白浓度与血管内皮生长因子浓度显著相关（相关系数，R = 0.78，P<0.001）。