番荔枝（刺果番荔枝）乙醇提取物和水提取物的植物化学筛选、抗氧化活性及体外抗癌潜力

Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola).

作者信息

Gavamukulya Yahaya, Abou-Elella Faten, Wamunyokoli Fred, AEl-Shemy Hany

机构信息

Molecular Biology and Biotechnology Department, Pan African University, Institute for Basic Sciences, Technology and Innovation (PAUISTI - JKUAT), Nairobi, Kenya.

Biochemistry Department, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt.

出版信息

Asian Pac J Trop Med. 2014 Sep;7S1:S355-63. doi: 10.1016/S1995-7645(14)60258-3.

DOI:10.1016/S1995-7645(14)60258-3

PMID:25312150

Abstract

OBJECTIVE

To determine the phytochemical composition, antioxidant and anticancer activities of ethanolic and water leaves extracts of Annona muricata (A. muricata) from the Eastern Uganda.

METHODS

Phytochemical screening was conducted using standard qualitative methods and a Chi-square goodness of fit test was used to assign the relative abundance of the different phytochemicals. The antioxidant activity was determined using the 2, 2-diphenyl-2-picrylhydrazyl and reducing power methods whereas the in vitro anticancer activity was determined using three different cell lines.

RESULTS

Phytochemical screening of the extracts revealed that they were rich in secondary class metabolite compounds such as alkaloids, saponins, terpenoids, flavonoids, coumarins and lactones, anthraquinones, tannins, cardiac glycosides, phenols and phytosterols. Total phenolics in the water extract were (683.69±0.09) μg/mL gallic acid equivalents (GAE) while it was (372.92±0.15) μg/mL GAE in the ethanolic extract. The reducing power was 216.41 μg/mL in the water extract and 470.51 μg/mL GAE in the ethanolic extract. In vitro antioxidant activity IC50 was 2.0456 mg/mL and 0.9077 mg/mL for ethanolic and water leaves extracts of A. muricata respectively. The ethanolic leaves extract was found to be selectively cytotoxic in vitro to tumor cell lines (EACC, MDA and SKBR3) with IC50 values of 335.85 μg/mL, 248.77 μg/mL, 202.33 μg/mL respectively, while it had no cytotoxic effect on normal spleen cells. The data also showed that water leaves extract of A. muricata had no anticancer effect at all tested concentrations.

CONCLUSIONS

The results showed that A. muricata was a promising new antioxidant and anticancer agent.

摘要

目的

测定乌干达东部刺果番荔枝（A. muricata）乙醇提取物和水提取物的植物化学成分、抗氧化和抗癌活性。

方法

采用标准定性方法进行植物化学筛选，并使用卡方拟合优度检验来确定不同植物化学成分的相对丰度。使用2,2-二苯基-1-苦基肼自由基清除法和还原能力法测定抗氧化活性，而体外抗癌活性则使用三种不同的细胞系进行测定。

结果

提取物的植物化学筛选显示，它们富含次生代谢物化合物，如生物碱、皂苷、萜类、黄酮类、香豆素和内酯、蒽醌、单宁、强心苷、酚类和植物甾醇。水提取物中的总酚含量为（683.69±0.09）μg/mL没食子酸当量（GAE），而乙醇提取物中的总酚含量为（372.92±0.15）μg/mL GAE。水提取物的还原能力为216.41 μg/mL，乙醇提取物的还原能力为470.51 μg/mL GAE。刺果番荔枝乙醇叶提取物和水叶提取物的体外抗氧化活性IC50分别为2.0456 mg/mL和0.9077 mg/mL。发现乙醇叶提取物在体外对肿瘤细胞系（EACC、MDA和SKBR3）具有选择性细胞毒性，IC50值分别为335.85 μg/mL、248.77 μg/mL、202.33 μg/mL，而对正常脾细胞无细胞毒性作用。数据还表明，刺果番荔枝水叶提取物在所有测试浓度下均无抗癌作用。