商业植物产品——南非叶（夹竹桃科）的体外抗HIV及抗氧化活性

In vitro anti-HIV and antioxidant activity of Hoodia gordonii (Apocynaceae), a commercial plant product.

作者信息

Kapewangolo Petrina, Knott Michael, Shithigona Regina E K, Uusiku Sylvia L, Kandawa-Schulz Martha

机构信息

Department of Chemistry and Biochemistry, Faculty of Science, University of Namibia, P/Bag 13301, Windhoek, Namibia.

School of Pharmacy, University of Namibia, P/Bag 13301, Windhoek, Namibia.

出版信息

BMC Complement Altern Med. 2016 Oct 24;16(1):411. doi: 10.1186/s12906-016-1403-7.

DOI:10.1186/s12906-016-1403-7

PMID:27776523

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5078957/

Abstract

BACKGROUND

Hoodia gordonii products are widely commercialized for anti-obesity purposes; however, minimal research is available on the other health properties demonstrated by this popular herbal plant.

METHODS

H. gordonii crude extracts (ethanol and ethyl acetate) were assayed for in vitro anti-HIV-1 protease (PR), reverse transcriptase (RT) and integrase activity. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assays were used for the antioxidant analysis. In addition, qualitative and quantitative phytochemical analyses of the extracts were determined using standard methods.

RESULTS

H. gordonii extract demonstrated good inhibition against HIV RT with IC values of 73.55 ± 0.04 and 69.81 ± 9.45 μg/mL for ethanol and ethyl acetate extracts, respectively. Both extracts also demonstrated inhibitory activity against HIV PR with IC values of 97.29 ± 0.01 and 63.76 ± 9.01 μg/mL for ethanol and ethyl acetate extracts. In addition, H. gordonii also showed good antioxidant activity with IC values of 124.6 ± 11.3 and 126.2 ± 3.15 μg/mL obtained for ethanol and ethyl acetate extracts, respectively. The reducing power of H. gordonii extracts increased as the concentration increased which confirmed the presence of antioxidants (reductants) in the extracts. Phytochemical screening of H. gordonii revealed the presence of phenolics, alkaloids, terpenes, steroids, cardiac glycosides and tannins in the ethanolic extract, while the ethyl acetate extract only showed the presence of phenolics, cardiac glycosides and steroids. The total phenolic content was 420 ± 0.17 and 319.9 ± 0.2 mg GAE/g for the ethanol and ethyl acetate extracts, respectively. The ethanol extract, which revealed the presence of tannins, had a tannin content of 330 ± 0.2 mg TAE/g extract.

CONCLUSION

This data suggests that H. gordonii has good in vitro inhibition against selected HIV-1 enzymes as well as antioxidant properties, suggesting new potential uses for this commercial plant.

摘要

背景

蝴蝶亚仙人掌产品因具有抗肥胖功效而被广泛商业化；然而，对于这种广受欢迎的草药植物所展现出的其他健康特性，相关研究却极为有限。

方法

对蝴蝶亚仙人掌粗提取物（乙醇提取物和乙酸乙酯提取物）进行体外抗HIV-1蛋白酶（PR）、逆转录酶（RT）及整合酶活性检测。采用2,2-二苯基-1-苦基肼（DPPH）法和还原力测定法进行抗氧化分析。此外，运用标准方法对提取物进行定性和定量植物化学分析。

结果

蝴蝶亚仙人掌提取物对HIV RT表现出良好的抑制作用，乙醇提取物和乙酸乙酯提取物的IC值分别为73.55±0.04和69.81±9.45μg/mL。两种提取物对HIV PR也均有抑制活性，乙醇提取物和乙酸乙酯提取物的IC值分别为97.29±0.01和63.76±9.01μg/mL。此外，蝴蝶亚仙人掌还展现出良好的抗氧化活性，乙醇提取物和乙酸乙酯提取物的IC值分别为124.6±11.3和126.2±3.15μg/mL。蝴蝶亚仙人掌提取物的还原力随浓度增加而增强，这证实了提取物中存在抗氧化剂（还原剂）。对蝴蝶亚仙人掌进行植物化学筛选发现，乙醇提取物中含有酚类、生物碱、萜类、甾体、强心苷和单宁，而乙酸乙酯提取物仅含有酚类、强心苷和甾体。乙醇提取物和乙酸乙酯提取物的总酚含量分别为420±0.17和319.9±0.2mg GAE/g。检测出含有单宁的乙醇提取物，其单宁含量为330±0.2mg TAE/g提取物。