A comprehensive review on perfusion method development for bone marrow collection and stem cell transplantation.

Bone marrow transplant (BMT) is done by the replacement of damaged bone marrow with healthy one. These healthy bone marrow cells (BMCs) are usually collected from the crest of the Ilium in humans hence these cells are used to replace damaged ones in the treatment of bone marrow related diseases such as leukemia, aplastic anemia, congenital immunodeficiency and autoimmune diseases. Even though there are different methods, perfusion method is one of the simple, safe and less contaminated methods used to harvest BMCs and it can reduce the risk in allogenic BMT. Intra bone marrow-bone marrow transfer (IBM-BMT) is one of the best procedures for allogenic BMT. Due to enlisting of hematopoietic stem cells and mesenchymal stem cells, which are derived from donor, this method has distinguishable advantages in allogenic BMT. In this paper the perfusion method (for harvesting BMCs) and IBM-BMT (for their transplantation) have been critically reviewed and showed that both methods are together will become an effective combination in allogeneic BMT.