State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences , Dong_Nanhu Road 3888, Changchun, Jilin 130033, P. R. China.
ACS Appl Mater Interfaces. 2014;6(21):19327-35. doi: 10.1021/am505417v. Epub 2014 Oct 27.
Nanotechnology, as a double-edged sword, endows gold nanoparticles (GNPs) more "power" in bioimaging and theragnostics, whereas an outstanding issue associated with the biocompatibility of GNPs should also be addressed. Especially for the silica-coated gold nanospheres (GNSs) and gold nanorods (GNRs), there is increasing attention to explore the application, because the surface silica encapsulation has been proved to be an alternative strategy for other organic surface coatings. However, among those reports there are very limited publications to focus on the toxicity of silica-coated GNSs and GNRs. Besides, the existing detoxification methods via surface chemistry on GNPs greatly improve the biocompatibility but still undergo challenges for high dose (>100 pM) demand and long-term stability. Here, we demonstrated a straightforward, low-cost, universal strategy for the surface chemistry on GNPs via silica encapsulating. Different size, shape, dose, and surface capping of GNPs for the nanotoxicity test have been carefully discussed. After silica encapsulating, the detoxification for all GNPs presents significantly from HepG2 cell proliferation results, especially for the GNRs. This new straightforward strategy will definitely rationalize the biocompatibility issue of GNPs and also provide potential for other surface chemistry methodology in biomedical fields.
纳米技术是一把双刃剑，为金纳米粒子（GNPs）在生物成像和治疗学方面赋予了更多的“能力”，但同时也应该解决与 GNPs 生物相容性相关的突出问题。特别是对于涂覆有二氧化硅的金纳米球（GNSs）和金纳米棒（GNRs），人们越来越关注其应用，因为表面二氧化硅封装已被证明是替代其他有机表面涂层的一种策略。然而，在这些报道中，很少有出版物专门关注涂覆有二氧化硅的 GNSs 和 GNRs 的毒性。此外，通过表面化学方法对 GNPs 进行解毒，虽然大大提高了其生物相容性，但仍面临高剂量（>100 pM）和长期稳定性的挑战。在这里，我们通过二氧化硅封装，展示了一种简单、低成本、通用的 GNPs 表面化学处理策略。我们仔细讨论了不同尺寸、形状、剂量和表面封端的 GNPs 纳米毒性测试。经过二氧化硅封装后，所有 GNPs 的解毒效果都从 HepG2 细胞增殖结果中得到了显著改善，特别是对 GNRs 而言。这种新的简单策略将肯定能够合理解决 GNPs 的生物相容性问题，并为生物医学领域的其他表面化学方法提供潜力。
