Yandle T G, Brennan S O, Espiner E A, Nicholls M G, Richards A M
Department of Endocrinology, Princess Margaret Hospital, Christchurch, New Zealand.
Peptides. 1989 Jul-Aug;10(4):891-4. doi: 10.1016/0196-9781(89)90131-9.
We previously demonstrated the presence of ANF(99-126), and ANF(99-126) cleaved between Cys105 and Phe106 (cleaved ANF), in human coronary sinus plasma. We now report that cleaved ANF is formed when synthetic ANF(99-126) is added to human plasma. When synthetic ANF(99-126) was incubated in heparinized human plasma, HPLC analysis showed two degradation products. The main product was shown by amino acid and sequence analysis to be cleaved ANF. Degradation of ANF was inhibited by EDTA and phosphoramidon. These findings are consistent with the action of endopeptidase EC 3.4.24.11, which may play an important part in the biological inactivation of ANF.
我们之前已证实在人冠状窦血浆中存在心房钠尿肽（ANF(99 - 126)）以及在半胱氨酸105和苯丙氨酸106之间裂解的ANF(99 - 126)（裂解型ANF）。我们现在报告，当将合成的ANF(99 - 126)添加到人血浆中时会形成裂解型ANF。当合成的ANF(99 - 126)在肝素化的人血浆中孵育时，高效液相色谱分析显示有两种降解产物。通过氨基酸和序列分析表明主要产物是裂解型ANF。ANF的降解受到乙二胺四乙酸（EDTA）和磷酰胺脒的抑制。这些发现与内肽酶EC 3.4.24.11的作用一致，该酶可能在ANF的生物失活中起重要作用。
