Çabuş N, Oğuz E O, Tufan A Ç, Adıgüzel E
Department of Histology and Embryology, Faculty of Medicine, Hacettepe University , Sihhiye Ankara , Turkey.
Biotech Histochem. 2015 Feb;90(2):132-9. doi: 10.3109/10520295.2014.965277. Epub 2014 Oct 14.
Aluminium has toxic effects on many organ systems of the human body. Aluminium toxicity also is a factor in many neurodegenerative diseases. We investigated changes in numbers of hippocampal neurons in rats exposed to aluminium using an optical fractionator and we investigated aluminium-induced apoptosis using the transferase mediated dUTP nick end labeling (TUNEL) assay. Twenty-four female rats were divided equally into control, sham and aluminium-exposed groups. The control group received no treatment. The two treatment groups were injected intraperitoneally with 1 ml 0.9% saline without (sham) and with 3 mg/ml aluminium sulfate every day for two weeks. Following the treatments, the brains were removed, the left hemisphere was used for hippocampal neuron counting using an optical fractionator and the right hemisphere was investigated using hippocampal TUNEL assay to determine the apoptotic index. The number of neurons in the stratum pyramidale of the hippocampus was significantly less in the aluminium group than in the control and sham groups; there was no significant difference between the control and sham groups. The apoptotic index also was significantly higher in the aluminium group than in the other two groups. We quantified the toxic effects of aluminium on the rat hippocampus and determined that apoptosis was the mechanism of aluminium-induced neuron death in the hippocampus.
铝对人体的许多器官系统都有毒性作用。铝中毒也是许多神经退行性疾病的一个因素。我们使用光学分割器研究了暴露于铝的大鼠海马神经元数量的变化，并使用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法研究了铝诱导的细胞凋亡。将24只雌性大鼠平均分为对照组、假手术组和铝暴露组。对照组不接受任何处理。两个处理组每天腹腔注射1 ml 0.9%生理盐水（假手术组不添加，铝暴露组添加3 mg/ml硫酸铝），持续两周。处理后，取出大脑，左半球用于通过光学分割器计数海马神经元，右半球用于通过海马TUNEL法检测以确定凋亡指数。铝暴露组海马锥体细胞层的神经元数量显著少于对照组和假手术组；对照组和假手术组之间无显著差异。铝暴露组的凋亡指数也显著高于其他两组。我们量化了铝对大鼠海马的毒性作用，并确定细胞凋亡是铝诱导海马神经元死亡的机制。
