对正在接受康复治疗的蠵龟（Caretta caretta）血红蛋白结合蛋白的评估。

Assessment of hemoglobin binding protein in loggerhead sea turtles (Caretta caretta) undergoing rehabilitation.

作者信息

Dickey Meranda, Cray Carolyn, Norton Terry, Murray Maureen, Barysauskas Constance, Arheart Kristopher L, Nelson Steven, Rodriguez Marilyn

出版信息

J Zoo Wildl Med. 2014 Sep;45(3):700-3. doi: 10.1638/2013-0262R1.1.

DOI:10.1638/2013-0262R1.1

PMID:25314847

Abstract

The acute phase response is an important component of the early reaction of the immune system to insults including infection, inflammation, trauma, neoplasia, and stress. Acute phase proteins are valuable prognostic indicators in many mammalian species but have been poorly studied in reptiles thus far. This study examined 18 paired samples from loggerhead sea turtles (Caretta caretta) for changes observed during the rehabilitation period. Analyses performed included packed cell volume (PCV), hemoglobin binding protein, and plasma protein electrophoresis. Significant differences were observed in all of the protein electrophoresis values. Notably, the concentration of hemoglobin binding protein (as determined by a haptoglobin assay) increased in conjunction with rising total protein (by refractometry) and PCV. The results indicate that this assay may have the potential to be a useful tool in assessing the health of sea turtles.

摘要

急性期反应是免疫系统对包括感染、炎症、创伤、肿瘤和应激在内的损伤作出早期反应的重要组成部分。急性期蛋白在许多哺乳动物物种中是有价值的预后指标，但迄今为止在爬行动物中研究较少。本研究检查了18对蠵龟（Caretta caretta）康复期样本的变化情况。进行的分析包括红细胞压积（PCV）、血红蛋白结合蛋白和血浆蛋白电泳。所有蛋白质电泳值均观察到显著差异。值得注意的是，血红蛋白结合蛋白的浓度（通过触珠蛋白测定法测定）随着总蛋白（通过折射法测定）和红细胞压积的升高而增加。结果表明，该检测方法可能有潜力成为评估海龟健康状况的有用工具。