Fargnoli A S, Katz M G, Williams R D, Margulies K B, Bridges Charles R
Sanger Heart & Vascular Institute, Thoracic and Cardiac Surgery, Cannon Research Center, Carolinas Healthcare System, 1542 Garden Terrace, Charlotte, NC, 28203, USA.
J Cardiovasc Transl Res. 2014 Nov;7(8):756-67. doi: 10.1007/s12265-014-9593-1. Epub 2014 Oct 15.
This study evaluates needleless liquid jet method and compares it with three common experimental methods: (1) intramuscular injection (IM), (2) left ventricular intracavitary infusion (LVIC), and (3) LV intracavitary infusion with aortic and pulmonary occlusion (LVIC-OCCL). Two protocols were executed. First (n = 24 rats), retention of dye was evaluated 10 min after delivery in an acute model. The acute study revealed the following: significantly higher dye retention (expressed as % myocardial cross-section area) in the left ventricle in both the liquid jet [52 ± 4] % and LVIC-OCCL [58 ± 3] % groups p < 0.05 compared with IM [31 ± 8] % and LVIC [35 ± 4] %. In the second (n = 16 rats), each animal received adeno-associated virus encoding green fluorescent protein (AAV.EGFP) at a single dose with terminal 6-week endpoint. In the second phase with AAV.EGFP at 6 weeks post-delivery, a similar trend was found with liquid jet [54 ± 5] % and LVIC-OCCL [60 ± 8] % featuring more LV expression as compared with IM [30 ± 9] % and LVIC [23 ± 9] %. The IM and LVIC-OCCL cross sections revealed myocardial fibrosis. With more detailed development in future model studies, needleless liquid jet delivery offers a promising strategy to improve direct myocardial delivery.
本研究评估了无针液体喷射法，并将其与三种常见的实验方法进行比较：（1）肌肉注射（IM），（2）左心室内输注（LVIC），以及（3）伴有主动脉和肺动脉闭塞的左心室内输注（LVIC - OCCL）。执行了两个方案。首先（n = 24只大鼠），在急性模型中，于给药后10分钟评估染料保留情况。急性研究结果如下：与IM组[31±8]%和LVIC组[35±4]%相比，液体喷射组[52±4]%和LVIC - OCCL组[58±3]%的左心室染料保留率（以心肌横截面积百分比表示）显著更高，p < 0.05。在第二个方案（n = 16只大鼠）中，每只动物接受单剂量编码绿色荧光蛋白的腺相关病毒（AAV.EGFP），终点为6周。在给予AAV.EGFP后6周的第二阶段，发现了类似的趋势，液体喷射组[54±5]%和LVIC - OCCL组[60±8]%在左心室中的表达高于IM组[30±9]%和LVIC组[23±9]%。IM组和LVIC - OCCL组的横截面显示有心肌纤维化。随着未来模型研究的更详细发展，无针液体喷射给药提供了一种改善直接心肌给药的有前景的策略。
