Gustafsson Ove J R, Arentz Georgia, Hoffmann Peter
Adelaide Proteomics Centre, School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, Australia 5005.
Adelaide Proteomics Centre, School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, Australia 5005.
Biochim Biophys Acta. 2015 Jun;1854(6):559-80. doi: 10.1016/j.bbapap.2014.10.003. Epub 2014 Oct 12.
Retrospective proteomic studies, including those which aim to elucidate the molecular mechanisms driving cancer, require the assembly and characterization of substantial patient tissue cohorts. The difficulty of maintaining and accessing native tissue archives has prompted the development of methods to access archives of formalin-fixed tissue. Formalin-fixed tissue archives, complete with patient meta data, have accumulated for decades, presenting an invaluable resource for these retrospective studies. This review presents the current knowledge concerning formalin-fixed tissue, with descriptions of the mechanisms of formalin fixation, protein extraction, top-down proteomics, bottom-up proteomics, quantitative proteomics, phospho- and glycoproteomics as well as imaging mass spectrometry. Particular attention has been given to the inclusion of proteomic investigations of archived tumour tissue. This article is part of a Special Issue entitled: Medical Proteomics.
回顾性蛋白质组学研究，包括那些旨在阐明驱动癌症的分子机制的研究，需要组建并表征大量患者组织队列。维护和获取原始组织档案存在困难，这促使人们开发了从福尔马林固定组织档案中获取样本的方法。带有患者元数据的福尔马林固定组织档案已经积累了数十年，为这些回顾性研究提供了宝贵资源。本综述介绍了有关福尔马林固定组织的现有知识，描述了福尔马林固定的机制、蛋白质提取、自上而下蛋白质组学、自下而上蛋白质组学、定量蛋白质组学、磷酸化和糖蛋白质组学以及成像质谱。特别关注了存档肿瘤组织的蛋白质组学研究。本文是名为“医学蛋白质组学”的特刊的一部分。
