Lam Han Yuen, Yusoff Khatijah, Yeap Swee Keong, Subramani Tamilselvan, Abd-Aziz Suraini, Omar Abdul Rahman, Alitheen Noorjahan Banu
1. Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
2. Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Int J Med Sci. 2014 Sep 19;11(12):1240-7. doi: 10.7150/ijms.8170. eCollection 2014.
Immunotherapy has raised the attention of many scientists because it hold promise to be an attractive therapeutic strategy to treat a number of disorders. In this study, the immunomodulatory effects of low titers of Newcastle disease virus (NDV) AF2240 on human peripheral blood mononuclear cells (PBMC) were analyzed. We evaluated cytokine secretion and PBMC activation by cell proliferation assay, immunophenotyping and enzyme linked immunosorbent assay. The proliferation of the human PBMC was measured to be 28.5% and 36.5% upon treatment with 8 hemaglutinin unit (HAU) and 2 HAU of NDV respectively. Interestingly, the percentage of cells with activating markers CD16 and CD56 were increased significantly. Furthermore, the intracellular perforin and granzyme levels were also increased upon virus infection. Human PBMC treated with NDV titer 8 HAU was found to stimulate the highest level of cytokine production including interferon-γ, interleukin-2 and interleukin-12. The release of these proteins contributes to the antitumor effect of PBMC against MCF-7 breast cancer cells. Based on the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay, activated human PBMC showed high cytolytic efficiency towards human breast tumor cells. In summary, NDV was able to stimulate PBMC proliferation, cytokine secretion and cytolytic activity.
免疫疗法已引起众多科学家的关注，因为它有望成为治疗多种疾病的一种有吸引力的治疗策略。在本研究中，分析了低滴度新城疫病毒（NDV）AF2240对人外周血单个核细胞（PBMC）的免疫调节作用。我们通过细胞增殖试验、免疫表型分析和酶联免疫吸附试验评估细胞因子分泌和PBMC激活情况。用8个血凝素单位（HAU）和2个HAU的NDV处理后人PBMC的增殖率分别为28.5%和36.5%。有趣的是，具有激活标记CD16和CD56的细胞百分比显著增加。此外，病毒感染后细胞内穿孔素和颗粒酶水平也升高。发现用8 HAU滴度的NDV处理的人PBMC刺激产生包括干扰素-γ、白细胞介素-2和白细胞介素-12在内的细胞因子的水平最高。这些蛋白质的释放有助于PBMC对MCF-7乳腺癌细胞的抗肿瘤作用。基于3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑试验，活化的人PBMC对人乳腺肿瘤细胞显示出高细胞溶解效率。总之，NDV能够刺激PBMC增殖、细胞因子分泌和细胞溶解活性。
