Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, UPM, Serdang, Malaysia.
Intervirology. 2010;53(2):87-94. doi: 10.1159/000264198. Epub 2009 Dec 3.
BACKGROUND/AIMS: Newcastle disease virus (NDV) is an avian paramyxovirus that has gained a lot of interest in cancer viro-therapeutic applications because of its ability to selectively induce apoptosis in human cancer cells. However, the underlying mechanisms by which NDV induces apoptosis in human cancer cells are still not entirely understood.
In this study we examined the effect of a Malaysian velogenic strain of NDV, known as AF2240, on some elements of the intrinsic pathway of apoptosis.
We show that NDV infection leads to conformational change of Bax protein. This is associated with the translocation of Bax from the cytoplasm to mitochondria and the release of cytochrome c into the cytoplasm. Interestingly, the level of Bcl-2 protein was not affected by NDV treatment.
We have shown that Bax conformational change and subcellular distribution is involved in the intrinsic pathway of apoptosis induced by NDV.
背景/目的：新城疫病毒（NDV）是一种禽副黏病毒，由于其能够选择性地诱导人类癌细胞凋亡，因此在癌症病毒治疗应用方面引起了广泛关注。然而，NDV 诱导人类癌细胞凋亡的潜在机制尚不完全清楚。
在本研究中，我们研究了一种马来西亚强毒力的 NDV 株，即 AF2240，对凋亡内在途径的某些元素的影响。
我们表明，NDV 感染导致 Bax 蛋白构象改变。这与 Bax 从细胞质到线粒体的易位以及细胞色素 c 释放到细胞质有关。有趣的是，NDV 处理并未影响 Bcl-2 蛋白的水平。
我们已经表明，Bax 构象改变和亚细胞分布参与了 NDV 诱导的凋亡内在途径。
