Basic Medicine College, Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu Province, China.
Henan Clinical Research Center of AIDS Controlled with Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450003, Henan Province, China.
Neural Regen Res. 2012 Dec 25;7(36):2867-73. doi: 10.3969/j.issn.1673-5374.2012.36.003.
In the present study, a human neuroblastoma cell line (SH-SY5Y) and BV-2 microglia were treated with amyloid-β peptide (25-35), as a model of Alzheimer's disease, to evaluate the protective effects of 10-3-10-8 g/mL Lingguizhugan decoction and to examine the underlying anti-inflammatory mechanism. Lingguizhugan decoction significantly enhanced the viability of SH-SY5Y cells with amyloid-β peptide-induced injury, and lowered levels of interleukin-1β, interleukin-6, tumor necrosis factor-α and nitric oxide in the culture supernatant of activated BV-2 microglia. The effects of 10-3 g/mL Lingguizhugan decoction were more significant. These results suggest that Lingguizhugan decoction can protect SH-SY5Y cells against amyloid-β peptide (25-35)-induced injury in a dose-dependent manner by inhibiting overexpression of inflammatory factors by activated microglia.
在本研究中，用人神经母细胞瘤细胞系 (SH-SY5Y) 和 BV-2 小胶质细胞用淀粉样β肽 (25-35) 处理，作为阿尔茨海默病的模型，以评估灵骨芝肝汤 10-3-10-8 g/ml 的保护作用，并探讨其潜在的抗炎机制。灵骨芝肝汤显著增强了淀粉样β肽诱导损伤的 SH-SY5Y 细胞的活力，并降低了活化的 BV-2 小胶质细胞培养上清液中白细胞介素-1β、白细胞介素-6、肿瘤坏死因子-α和一氧化氮的水平。10-3 g/ml 灵骨芝肝汤的作用更为显著。这些结果表明，灵骨芝肝汤可通过抑制活化小胶质细胞过度表达炎症因子，以剂量依赖的方式保护 SH-SY5Y 细胞免受淀粉样β肽 (25-35) 诱导的损伤。
