Shpylchyn V V, Antonyuk M Z, Ternovska T K
Tsitol Genet. 2014 Sep-Oct;48(5):43-53.
Changes in plant genomes of hybrid origin which become apparent on different levels of phenotypic manifestation of genetic and epigenetic changes are an important object of cytogenetics and molecular genetics. The changes in expression of the glaucousness trait in the artificial Triticinae amphidiploid Aurotica (AABBTT) were investigated; haplotypes of plants with the appearance of glaucous and non-glaucous genes were determined by hybridization experiments. It was supported that the changes in expression can be explained by (epi)mutations abolishing the efficiency of dominant alleles of orthologous series of glaucous inhibitor gene Iw. Analysis of F2 population with SSR markers specific to 2B and 2D wheat chromosomes and 2T chromosome of Aegilops mutica manifested associated inheritance of the Wms 102 and Wms 702 loci, which mapped to the short arm of chromosome 2D. The Wms702 marker was linked to the Iw2(T) gene and can now be used to detect that gene.
杂种起源的植物基因组变化在遗传和表观遗传变化的不同表型表现水平上变得明显,是细胞遗传学和分子遗传学的重要研究对象。研究了人工小麦族双二倍体Aurotica(AABBTT)中白粉性状表达的变化;通过杂交实验确定了出现白粉和无白粉基因的植物单倍型。研究结果支持了表达变化可由(表观)突变解释,这些突变消除了白粉抑制基因Iw直系同源系列显性等位基因的效率。利用特异于小麦2B和2D染色体以及节节麦2T染色体的SSR标记对F2群体进行分析,结果表明Wms 102和Wms 702位点存在连锁遗传,这两个位点定位于2D染色体短臂。Wms702标记与Iw2(T)基因连锁,现在可用于检测该基因。