Chiu Norman H L, Jia Zhenquan, Diaz Reynaldo, Wright Petra
Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, North Carolina, USA; Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, Greensboro, North Carolina, USA.
Environ Toxicol Chem. 2015 Jan;34(1):161-6. doi: 10.1002/etc.2774. Epub 2014 Nov 17.
Changes in protein expression as a cellular response to chemical exposure have been well established. Current methods for monitoring cellular responses usually require the use of specific reagents and/or labor-intensive procedures. The present study demonstrates the concept of using mass spectral pattern to distinguish different cellular responses. The concept is based on the ability to acquire a unique mass spectral pattern directly from a specific cell culture by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The results demonstrate that distinguishable and reproducible spectral patterns can be obtained from different cellular responses.
作为细胞对化学物质暴露的反应，蛋白质表达的变化已得到充分证实。目前监测细胞反应的方法通常需要使用特定试剂和/或劳动密集型程序。本研究展示了利用质谱模式区分不同细胞反应的概念。该概念基于通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）直接从特定细胞培养物中获取独特质谱模式的能力。结果表明，从不同细胞反应中可获得可区分且可重复的光谱模式。
