Clavijo Maria J, Oliveira Simone, Zimmerman Jeffrey, Rendahl Aaron, Rovira Albert
Department of Veterinary Population Medicine, College of Veterinary Medicine (Clavijo, Oliveira, Rovira), University of Minnesota, St. Paul, MNSchool of Statistics (Rendahl), University of Minnesota, St. Paul, MNDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA (Zimmerman).
Department of Veterinary Population Medicine, College of Veterinary Medicine (Clavijo, Oliveira, Rovira), University of Minnesota, St. Paul, MNSchool of Statistics (Rendahl), University of Minnesota, St. Paul, MNDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA (Zimmerman)
J Vet Diagn Invest. 2014 Nov;26(6):755-60. doi: 10.1177/1040638714555175. Epub 2014 Oct 15.
Mycoplasma hyorhinis has emerged as an important cause of systemic disease in nursery pigs. However, this bacterium can also be found in the upper respiratory tract of healthy swine. The current study describes the development of a quantitative polymerase chain reaction assay for the detection of M. hyorhinis and the evaluation of the assay in both disease diagnosis and disease surveillance using a large number of field samples. The analytical sensitivity was estimated to be 12 genome equivalents/μl. The assay was highly specific, detecting all 25 M. hyorhinis isolates tested and none of the 19 nontarget species tested. Assay repeatability was evaluated by testing different matrices spiked with known amounts of M. hyorhinis. Overall, assessment of the repeatability of the assay showed suitable precision within and between runs for all matrices. The coefficient of variation ranged from 10% to 24%. Mycoplasma hyorhinis DNA was detected in 48% of samples (pericardium, pleura, joints, nasal cavity, and lungs) from pigs with systemic disease. Mycoplasma hyorhinis was detected in nasal (92%) and oropharyngeal swabs (66%), as well as in oral fluids (100%). Potential uses of this tool involve the characterization of the prevalence of this pathogen in swine herds as well as bacterial quantification to evaluate intervention efficacy.
猪鼻支原体已成为保育猪全身性疾病的一个重要病因。然而，这种细菌也可在健康猪的上呼吸道中发现。本研究描述了一种用于检测猪鼻支原体的定量聚合酶链反应检测方法的开发，并使用大量现场样本对该检测方法在疾病诊断和疾病监测中的应用进行了评估。分析灵敏度估计为12个基因组当量/微升。该检测方法具有高度特异性，检测了所有25株受试猪鼻支原体分离株，而19株受试非靶标菌种均未检测到。通过检测添加已知量猪鼻支原体的不同基质来评估检测方法的重复性。总体而言，对该检测方法重复性的评估表明，所有基质在批次内和批次间均具有合适的精密度。变异系数在10%至24%之间。在患有全身性疾病的猪的48%的样本（心包、胸膜、关节、鼻腔和肺）中检测到了猪鼻支原体DNA。在鼻拭子（92%）和口咽拭子（66%）以及口腔液体（100%）中检测到了猪鼻支原体。该工具的潜在用途包括确定这种病原体在猪群中的流行特征以及进行细菌定量以评估干预效果。
