Rodda Luke N, Gerostamoulos Dimitri, Drummer Olaf H
Department of Forensic Medicine, Victorian Institute of Forensic Medicine, Monash University, 65 Kavanagh Street, Southbank, VIC, 3006, Australia.
Forensic Sci Med Pathol. 2014 Dec;10(4):550-6. doi: 10.1007/s12024-014-9609-9. Epub 2014 Oct 16.
Iso-α-acids (IAA) and reduced IAA can be used as beer-specific ingredient congeners to confirm beer consumption when detected in blood and other specimens using a UHPLC-MS/MS method. Recent analysis of postmortem casework demonstrated a high prevalence of beer consumption and the possibility of providing the source of alcohol in forensic casework. Research outlined in this manuscript has examined the degree to which the interval after death and quality of blood affects the concentration of IAA in postmortem cases. Postmortem whole blood and serum were analyzed in cases where natural or reduced IAA groups were detected. The trans-IAA, cis-IAA, and tetrahydro-IAA (TIAA) groups were subject to postmortem redistribution, although only weakly associated with the length of time from death to collection of specimens. Serum had threefold higher concentrations than blood for trans-IAA, cis-IAA, and TIAA. These studies confirm that although postmortem concentrations cannot be easily compared to concentrations found in living persons the presented findings do provide some understanding to assist in interpretation where the confirmation of beer consumption is required in forensic casework.
异α-酸（IAA）和还原型IAA可用作啤酒特有的成分同系物，当使用超高效液相色谱-串联质谱法（UHPLC-MS/MS）在血液和其他样本中检测到时，可用于确认啤酒摄入量。近期对死后案件工作的分析表明，啤酒消费的发生率很高，并且在法医案件工作中有可能提供酒精来源。本手稿中概述的研究考察了死后间隔时间和血液质量对死后案件中IAA浓度的影响程度。在检测到天然或还原型IAA组的案件中，对死后全血和血清进行了分析。反式-IAA、顺式-IAA和四氢-IAA（TIAA）组会发生死后再分布，尽管与从死亡到采集样本的时间长度仅有微弱关联。对于反式-IAA、顺式-IAA和TIAA，血清中的浓度比血液中的浓度高两倍。这些研究证实，尽管死后浓度不易与活人身上发现的浓度进行比较，但所呈现的研究结果确实有助于在法医案件工作中需要确认啤酒消费情况时进行解释。
