Li Donghai, Guo Jing, Zhou Xiumei, Chang Cheng, Zhang Shengxiang
a Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution , School of Life Sciences, Lanzhou University , Lanzhou , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1846-7. doi: 10.3109/19401736.2014.971253. Epub 2014 Oct 16.
The toad-headed lizards of genus Phrynocephalus are one of the most prevalent animals in the central Asian desert. A few studies have investigated molecular phylogenesis of Phrynocephalus, yet yield inconsistent results. Moreover, these studies were only based on a few specific DNA fragments of mitochondrial genome. To facilitate the clarification of molecular phylogenesis of Phrynocephalus, we conducted this study to sequence the entire mitochondrial genome of the Phrynocephalus helioscopus collected from Northwest China. The length of complete mitochondrial DNA is 16,249 nucleotides, consisting of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 2 control regions (CR). The gene arrangement and composition of P. helioscopus resemble those of other Phrynocephalus sand lizard, except for P. przewalskii and P. versicolor. The overall A, T, C, G base composition of the heavy-strand was 35.9%, 26.4%, 25.2%, 12.5%, respectively, which is biased toward AT (about 62.3%). The AT-biased base composition was similar to what observed in most vertebrates. The complete mitochondrial genome of P. helioscopus may help to clarify the phylogenetic relationships related to Phrynocephalus oviparity.
沙蜥属的蟾头蜥是中亚沙漠中最常见的动物之一。一些研究对沙蜥属的分子系统发育进行了调查，但结果并不一致。此外，这些研究仅基于线粒体基因组的一些特定DNA片段。为了便于阐明沙蜥属的分子系统发育，我们开展了本研究，对从中国西北部采集的荒漠沙蜥的整个线粒体基因组进行测序。完整线粒体DNA的长度为16,249个核苷酸，由13个蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因和2个控制区（CR）组成。除了西藏沙蜥和变色沙蜥外，荒漠沙蜥的基因排列和组成与其他沙蜥属沙蜥相似。重链的总体A、T、C、G碱基组成分别为35.9%、26.4%、25.2%、12.5%，偏向于AT（约62.3%）。这种偏向AT的碱基组成与大多数脊椎动物中观察到的情况相似。荒漠沙蜥的完整线粒体基因组可能有助于阐明与沙蜥属卵生相关的系统发育关系。
