Lu Hong-Wei, Dong Jia-Hong, Li Chong-Hui, Yu Qiang, Tang Wei
Department of General Surgery, Second Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an, China.
Clin Transplant. 2014 Oct;28(10):1202-8. doi: 10.1111/ctr.12419. Epub 2014 Oct 16.
To observe the morphologic changes in intrahepatic bile ducts and the defects of cholangiocyte primary cilia in patients with graft cholangiopathies.
Four patients who were diagnosed as graft cholangiopathies and underwent retransplantation were chosen as the study group; another four patients who underwent liver transplantation during the same period and recovered normally six months after the operation were the control group. The serum levels of biochemical indicators were measured, the morphologic changes in intrahepatic bile ducts and cholangiocyte primary cilia were observed, and the ciliary marker (α-tubulin) and membrane proteins (polycystin-1, TPPV4) were detected by immunofluorescence analysis and Western blot.
In the study group, biliary structures were vague and some bile ducts disappeared in portal areas; some epithelial cells were lost; lots of collagen was deposited and many phlogocytes infiltrated; microliths were found in some ductal lumens; partial biliary epithelial cells were necrosed; primary cilia and microvilli disappeared. In the control group, the structures of intrahepatic bile ducts and biliary epithelial cells were integrated and the primary cilia were present.
The morphologic changes in biliary epithelial cells and the defects of cholangiocyte primary cilia have a close correlation with graft cholangiopathies in liver transplantation.
观察移植肝胆管病患者肝内胆管的形态学变化及胆管上皮细胞原发性纤毛的缺陷。
选取4例诊断为移植肝胆管病并接受再次移植的患者作为研究组；同期另4例接受肝移植且术后6个月恢复正常的患者作为对照组。检测血清生化指标水平，观察肝内胆管及胆管上皮细胞原发性纤毛的形态学变化，采用免疫荧光分析和蛋白质免疫印迹法检测纤毛标志物（α-微管蛋白）和膜蛋白（多囊蛋白-1、TPPV4）。
研究组门管区胆管结构模糊，部分胆管消失；部分上皮细胞脱落；大量胶原沉积，有较多炎性细胞浸润；部分管腔内可见微结石；部分胆管上皮细胞坏死；原发性纤毛和微绒毛消失。对照组肝内胆管及胆管上皮细胞结构完整，有原发性纤毛。
胆管上皮细胞的形态学变化及胆管上皮细胞原发性纤毛的缺陷与肝移植中的移植肝胆管病密切相关。
