Malm Björn, Yoshimatsu Keiichi, Ye Lei, Krozer Anatol
Acreo Swedish ICT AB, Arvid Hedvallsbacke 4, 41133, Göteborg, Sweden; Biothema AB, Handens stationsväg 17, 13640, Handen, Sweden.
J Mol Recognit. 2014 Dec;27(12):714-21. doi: 10.1002/jmr.2397.
We follow template-binding induced aggregation of nanoparticles enantioselectively imprinted against (S)-propranolol, and the non-imprinted ones, using photon correlation spectroscopy (dynamic light scattering). The method requires no separation steps. We have characterized binding of (R,S)-propranolol to the imprinted polymers and determined the degree of non-specificity by comparing the specific binding with the results obtained using non-imprinted nanoparticles. Using (S)-propranolol as a template for binding to (S)-imprinted nanoparticle, and (R)-propranolol as a non-specific control, we have determined range of concentrations where chiral recognition can be observed. By studying aggregation induced by three analytes related to propranolol, atenolol, betaxolol, and 1-amino-3-(naphthalen-1-yloxy)propan-2-ol, we were able to determine which parts of the template are involved in the specific binding, discuss several details of specific adsorption, and the structure of the imprinted site.
我们采用光子相关光谱法（动态光散射），对以（S）-普萘洛尔为对映体选择性印迹的纳米颗粒以及非印迹纳米颗粒的模板结合诱导聚集进行了研究。该方法无需分离步骤。我们已对（R,S）-普萘洛尔与印迹聚合物的结合进行了表征，并通过将特异性结合与使用非印迹纳米颗粒获得的结果进行比较，确定了非特异性程度。以（S）-普萘洛尔作为与（S）-印迹纳米颗粒结合的模板，以（R）-普萘洛尔作为非特异性对照，我们确定了可观察到手性识别的浓度范围。通过研究与普萘洛尔、阿替洛尔、倍他洛尔和1-氨基-3-（萘-1-基氧基）丙-2-醇相关的三种分析物诱导的聚集，我们能够确定模板的哪些部分参与特异性结合，讨论特异性吸附的几个细节以及印迹位点的结构。
