Iversen Per Ole, Sioud Mouldy
Department of Nutrition, Oslo University Hospital, Norway, Montebello, 0310, Norway.
Methods Mol Biol. 2015;1218:263-8. doi: 10.1007/978-1-4939-1538-5_15.
Vaccination represents one the most effective methods of preventing disease. Because dendritic cells (DCs) are the most efficient antigen presenting cells, exploiting their plasticity is likely to yield improved therapeutic vaccines. Herein, we applied a novel DC-based vaccine (i.e., DC loaded with leukemia antigens that have been transfected with an IL-10 siRNA capable of coordinately activating DCs via TLR7/8) in a rat model of acute myeloid leukemia. Leukemic rats treated with this new vaccine had less leukemic cell mass in their bone marrows and less extramedullar dissemination of the leukemic disease examined postmortem compared with rats given the control vaccine. Collectively, the new strategy demonstrates the possible usefulness of dual siRNAs as an immunomodulatory drug with antileukemic properties.
疫苗接种是预防疾病最有效的方法之一。由于树突状细胞（DCs）是最有效的抗原呈递细胞，利用它们的可塑性可能会产生改良的治疗性疫苗。在此，我们在急性髓性白血病大鼠模型中应用了一种新型的基于DC的疫苗（即负载有白血病抗原的DC，该抗原已被能够通过TLR7/8协同激活DC的IL-10 siRNA转染）。与给予对照疫苗的大鼠相比，用这种新疫苗治疗的白血病大鼠骨髓中的白血病细胞团较少，死后检查的白血病疾病髓外扩散也较少。总的来说，新策略证明了双重siRNA作为具有抗白血病特性的免疫调节药物的潜在用途。
