Department of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University , Daegu, 702-701, South Korea.
Acta Histochem Cytochem. 2014 Jun 28;47(3):113-23. doi: 10.1267/ahc.14004. Epub 2014 Jun 26.
The purpose of this study was to investigate the localization of cells containing the calcium-binding proteins (CBPs) calbindin D28K (CB), calretinin (CR), and parvalbumin (PV) in the superior colliculus (SC) of the bat using immunocytochemistry. CB-immunoreactive (IR) cells formed a laminar tier within the upper superficial gray layer (SGL), while CR-IR cells were widely distributed within the optic layer (OL). Scattered CR-IR cells were also found within the intermediate gray, white, and deep gray layers. By contrast, PV-IR cells formed a laminar tier within the lower SGL and upper OL. Scattered PV-IR cells were also found throughout the intermediate layers, but without a specific laminar pattern. The CBP-IR cells varied in size and morphology: While most of the CB-IR cells in the superficial layers were small round or oval cells, most CR-IR cells in the intermediate and deep layers were large stellate cells. By contrast, PV-IR cells were small to large in size and included round or oval, stellate, vertical fusiform, and horizontal cells. The average diameters of the CB-, CR-, and PV-IR cells were 11.59, 17.17, and 12.60 μm, respectively. Double-immunofluorescence revealed that the percentage of co-localization with GABA-IR cells was 0.0, 0.0, and 10.27% of CB-, CR-, and PV-IR cells, respectively. These results indicate that CBP distribution patterns in the bat SC are unique compared with other mammalian SCs, which suggest functional diversity of these proteins in visually guided behaviors.
本研究旨在采用免疫细胞化学方法研究钙结合蛋白（CBPs）钙结合蛋白 D28K（CB）、钙调蛋白（CR）和副肌球蛋白（PV）在蝙蝠上丘（SC）中的细胞定位。CB-免疫反应（IR）细胞在上浅层灰色层（SGL）内形成层状层，而 CR-IR 细胞在视层（OL）内广泛分布。在中间灰色、白色和深灰色层中也发现了分散的 CR-IR 细胞。相比之下，PV-IR 细胞在下 SGL 和上 OL 内形成层状层。在中间层中也发现了分散的 PV-IR 细胞，但没有特定的层状模式。CBP-IR 细胞在大小和形态上存在差异：浅层的大多数 CB-IR 细胞为小圆或椭圆形细胞，而中间和深层的大多数 CR-IR 细胞为大星形细胞。相比之下，PV-IR 细胞的大小从小到大都有，包括圆形或椭圆形、星形、垂直梭形和水平细胞。CB-、CR-和 PV-IR 细胞的平均直径分别为 11.59、17.17 和 12.60μm。双重免疫荧光显示，与 GABA-IR 细胞共定位的百分比分别为 CB-、CR-和 PV-IR 细胞的 0.0、0.0 和 10.27%。这些结果表明，与其他哺乳动物 SC 相比，蝙蝠 SC 中 CBP 的分布模式是独特的，这表明这些蛋白质在视觉引导行为中具有功能多样性。
