Korneev A Ia, Bragin E O
Biull Eksp Biol Med. 1989 Oct;108(10):461-4.
3H vasopressin specifically binds to the binding sites in liver, kidney and adenohypophysis with Bmax = 11.8 +/- 5.6, 1.7 +/- 0.5 and 4.9 +/- 1.2 pmol/g tissue and Kd = 1.5 +/- 0.5, 0.66 +/- 0.21 and 0.84 +/- 0.21 nM, correspondingly. Specific binding increases in the presence of Mg2+ and Ni2+ and decreases at high temperature (37 degrees). The presence of high affinity binding sites for 3H vasopressin was shown in the rat adrenals, binding was saturable and reversible. Concentration of vasopressin binding sites in adrenals is 6-8 fold less than in adenohypophysis. It is supposed that vasopressin receptors in adrenals may participate in the regulation of corticosteroid secretion.
3H 血管加压素特异性结合于肝脏、肾脏和腺垂体中的结合位点，其最大结合容量（Bmax）分别为 11.8±5.6、1.7±0.5 和 4.9±1.2 pmol/g 组织，解离常数（Kd）分别为 1.5±0.5、0.66±0.21 和 0.84±0.21 nM。在 Mg2+和 Ni2+存在时特异性结合增加，在高温（37 摄氏度）时降低。在大鼠肾上腺中显示存在 3H 血管加压素的高亲和力结合位点，结合是可饱和且可逆的。肾上腺中血管加压素结合位点的浓度比腺垂体中的低 6 - 8 倍。推测肾上腺中的血管加压素受体可能参与皮质类固醇分泌的调节。
