Semenzato G, Trentin L, Zambello R, Agostini C, Chisesi T, Pizzolo G
Padua University School of Medicine, Department of Clinical Medicine, Italy.
Cancer Immunol Immunother. 1989;30(4):254-6. doi: 10.1007/BF01665013.
Experiments herein reported were designed to clarify the degree of sensitivity of hairy cells to lysis in vitro. The cytotoxic capacity of peripheral blood lymphocytes from hairy cell leukemia patients against autologous and/or allogenic hairy cells was tested both at resting conditions and after in vitro stimulation. While effectors activated by interferon alpha, lectins, or interleukin-2 were unable to induce a lysis of hairy cells, in some cases we were successful in eliciting a definite lysis of these cells by triggering the effector cells with anti-CD3 monoclonal antibodies. Our results favour the interpretation that hairy cells are relatively, but not completely resistant to in vitro lysis. Furthermore, the evidence that anti-CD3 antibodies increase the efficacy of the cytotoxic machinery might support the use of these molecules in designing new immunotherapeutic approaches against tumor targets.
本文报道的实验旨在阐明毛细胞在体外对裂解的敏感程度。对毛细胞白血病患者外周血淋巴细胞在静息状态及体外刺激后针对自体和/或同种异体毛细胞的细胞毒能力进行了检测。虽然由α干扰素、凝集素或白细胞介素-2激活的效应细胞无法诱导毛细胞裂解，但在某些情况下，我们通过用抗CD3单克隆抗体触发效应细胞成功地引发了这些细胞的明确裂解。我们的结果支持这样一种解释，即毛细胞对体外裂解具有相对但并非完全的抗性。此外，抗CD3抗体可提高细胞毒机制功效这一证据可能支持在设计针对肿瘤靶点的新免疫治疗方法中使用这些分子。
