Fujino T, Fukuda K, Hamajima F, Ishii Y
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Helminthol. 1989 Dec;63(4):315-27. doi: 10.1017/s0022149x00009214.
Histochemical tests were done on newly excysted metacercariae and worms recovered from an abnormal host (rat) and the definitive host (dog) for some oxidoreductases, phosphatases and glycosidases. The results demonstrate that rat worms have enzymatic distribution and intensities more similar to those of metacercariae than to adult worms from dogs. Ultracytochemical examination of acid and alkaline phosphatase and Mg-ATPase activity was also carried out. Acid phosphatase activity occurred exceptionally in the excretory bladder and caeca of dog worms. No activity was observed in rat worms except for lysosomal granules in the tegument. Alkaline phosphatase activity was exhibited in the excretory bladder in both dog and rat worms. Mg-ATPase activity occurred in the tegument and parenchymal cells in dog worms and in the excretory bladder in rat worms. In metacercariae, little or no reaction for these enzymes was present except for Mg-ATPase activity on the excretory ducts. These observations, together with the histochemical results, indicate that metabolic activity in rat worms is higher than in metacercariae although it is strongly reduced compared with dog worms.
对新脱囊的后尾蚴以及从异常宿主（大鼠）和终末宿主（犬）体内获取的虫体进行了一些氧化还原酶、磷酸酶和糖苷酶的组织化学检测。结果表明，大鼠体内的虫体酶分布和强度与后尾蚴更为相似，而非与犬的成虫相似。还进行了酸性和碱性磷酸酶以及镁 - 三磷酸腺苷酶活性的超微细胞化学检查。酸性磷酸酶活性特别出现在犬虫体的排泄囊和盲肠中。除了皮层中的溶酶体颗粒外，在大鼠虫体中未观察到活性。碱性磷酸酶活性在犬和大鼠虫体的排泄囊中均有表现。镁 - 三磷酸腺苷酶活性在犬虫体的皮层和实质细胞中出现，在大鼠虫体的排泄囊中出现。在后尾蚴中，除了排泄管上的镁 - 三磷酸腺苷酶活性外，这些酶几乎没有反应或没有反应。这些观察结果与组织化学结果一起表明，大鼠虫体的代谢活性高于后尾蚴，尽管与犬虫体相比其代谢活性大大降低。
