Wu Z W, Maddy A H
Department of Zoology, University of Edinburgh, U.K.
J Immunol Methods. 1989 Nov 30;124(2):165-9. doi: 10.1016/0022-1759(89)90349-9.
A method for the identification of antigens in a complex mixture of proteins is described. It exploits the effect which addition of a monoclonal antibody and resultant formation of antibody/antigen complexes has on the apparent retention time of the antigen on a size exclusion column. An antigen in the mixture can be identified as that component whose retention time is decreased by addition of its antibody. The principle of the method is demonstrated using an HPLC size exclusion column (DuPont GF450) and three MoAbs, PD7/26, DA6 147 and W6/32 which are directed against three antigens of the lymphocyte surface, the leucocyte-common antigen, class II MHC and class I MHC respectively.
本文描述了一种鉴定蛋白质复杂混合物中抗原的方法。该方法利用了添加单克隆抗体以及由此形成的抗体/抗原复合物对抗原在尺寸排阻柱上的表观保留时间的影响。混合物中的一种抗原可被鉴定为其保留时间因添加其抗体而缩短的组分。使用高效液相色谱尺寸排阻柱（杜邦GF450）和三种分别针对淋巴细胞表面三种抗原（白细胞共同抗原、II类主要组织相容性复合体和I类主要组织相容性复合体）的单克隆抗体PD7/26、DA6 147和W6/32证明了该方法的原理。
