Saito S, Fujii M, Saito M, Kato Y, Moriyama I, Ichijo M
Department of Obstetrics and Gynecology, Nara Medical University, Japan.
J Reprod Immunol. 1989 Sep;16(1):31-42. doi: 10.1016/0165-0378(89)90004-1.
Interleukin-2 receptors (IL-2R) on mononuclear cells from human umbilical cord blood were investigated after stimulation by phytohemagglutinin (PHA) and anti-CD3 antibody. The proportion of cells expressing the Tac antigen (IL-2R alpha, p55) did not differ from that for adult mononuclear cells. However, the levels of high affinity IL-2R (H-IL-2R) and low affinity IL-2R (L-IL-2R) present on the PHA blasts of cord blood cells were shown to be twice and three times, respectively, that of adults by interleukin-2 (IL-2) binding assay. The level of low affinity IL-2R in the cord blood cells was approximately double that of adult cells after activation by anti-CD3 antibody, but no difference was observed in the levels of high affinity IL-2R. Functional investigation of IL-2R revealed that the amount of internalized IL-2 in PHA-stimulated cord blood mononuclear cells was twice that in adult mononuclear cells but there were no differences between them in the time course of internalization and degradation. Although there were significant numbers of H-IL-2R in premature (25 and 28 weeks) infants following PHA stimulation, there were markedly fewer following anti-CD3 stimulation. Prematurity of the T-cell activation system through the CD3 pathway at this stage is therefore indicated.
在用植物血凝素（PHA）和抗CD3抗体刺激后，对来自人脐带血的单核细胞上的白细胞介素-2受体（IL-2R）进行了研究。表达Tac抗原（IL-2Rα，p55）的细胞比例与成人单核细胞的比例没有差异。然而，通过白细胞介素-2（IL-2）结合试验显示，脐带血细胞PHA刺激的母细胞上存在的高亲和力IL-2R（H-IL-2R）和低亲和力IL-2R（L-IL-2R）水平分别是成人的两倍和三倍。在用抗CD3抗体激活后，脐带血细胞中低亲和力IL-2R的水平约为成人细胞的两倍，但高亲和力IL-2R的水平未观察到差异。IL-2R的功能研究表明，PHA刺激的脐带血单核细胞中内化的IL-2量是成人单核细胞中的两倍，但它们在内化和降解的时间进程上没有差异。尽管在PHA刺激后早产（25和28周）婴儿中有大量的H-IL-2R，但在抗CD3刺激后明显较少。因此表明在这个阶段通过CD3途径的T细胞激活系统不成熟。
